Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Structure and mechanism of glutamate racemase from Aquifex pyrophilus
Autore:
Hwang, KY; Cho, CS; Kim, SS; Sung, HC; Yu, YG; Cho, YJ;
Indirizzi:
Korea Inst Sci & Technol, Struct Biol Ctr, Seoul 130650, South Korea KoreaInst Sci & Technol Seoul South Korea 130650 ul 130650, South Korea Korea Univ, Dept Biotechnol, Seoul 136701, South Korea Korea Univ Seoul South Korea 136701 iotechnol, Seoul 136701, South Korea
Titolo Testata:
NATURE STRUCTURAL BIOLOGY
fascicolo: 5, volume: 6, anno: 1999,
pagine: 422 - 426
SICI:
1072-8368(199905)6:5<422:SAMOGR>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
COMPLETE GENOME SEQUENCE; AMINO-ACID RACEMASE; MANDELATE RACEMASE; CATALYTIC RESIDUES; CRYSTAL-STRUCTURE; ALANINE RACEMASE; CARBON ACIDS; ACTIVE-SITE; PROTEIN; IDENTIFICATION;
Tipo documento:
Letter
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Cho, YJ Koreaeanst Sci & Technol, Struct Biol Ctr, POB 131, Seoul 130650, South Kor Korea Inst Sci & Technol POB 131 Seoul South Korea 130650 uth Kor
Citazione:
K.Y. Hwang et al., "Structure and mechanism of glutamate racemase from Aquifex pyrophilus", NAT ST BIOL, 6(5), 1999, pp. 422-426

Abstract

Glutamate racemase (MurI) is responsible for the synthesis of D-glutamate,an essential building block of the peptidoglycan layer in bacterial cell walls. The crystal structure of glutamate racemase from Aquifex pyrophilus, determined at 2.3 Angstrom resolution, reveals that the enzyme forms a dimer and each monomer consists of two alpha/beta fold domains, a unique structure that has not been observed in other racemases or members of an enolase superfamily, A substrate analog, D-glutamine, binds to the deep pocket formed by conserved residues from two monomers. The structural and mutational analyses allow us to propose a mechanism of metal cofactor-independent glutamate racemase in which two cysteine residues are involved in catalysis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 19:49:34