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Titolo:
COORDINATED INCORPORATION OF SKELETAL-MUSCLE DIHYDROPYRIDINE RECEPTORS AND RYANODINE RECEPTORS IN PERIPHERAL COUPLINGS OF BC(3)H1 CELLS
Autore:
PROTASI F; FRANZINIARMSTRONG C; FLUCHER BE;
Indirizzi:
UNIV PENN,SCH MED,DEPT CELL & DEV BIOL PHILADELPHIA PA 19104 INNSBRUCK UNIV,DEPT BIOCHEM PHARMACOL A-6020 INNSBRUCK AUSTRIA
Titolo Testata:
The Journal of cell biology
fascicolo: 4, volume: 137, anno: 1997,
pagine: 859 - 870
SICI:
0021-9525(1997)137:4<859:CIOSDR>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
CALCIUM-RELEASE CHANNEL; RETICULUM GLYCOPROTEIN TRIADIN; JUNCTIONAL FOOT PROTEIN; SARCOPLASMIC-RETICULUM; TRANSVERSE TUBULES; 1,4-DIHYDROPYRIDINE RECEPTOR; CHARGE MOVEMENT; MONOCLONAL-ANTIBODY; DYSGENIC MUSCLE; COMPLEX;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
64
Recensione:
Indirizzi per estratti:
Citazione:
F. Protasi et al., "COORDINATED INCORPORATION OF SKELETAL-MUSCLE DIHYDROPYRIDINE RECEPTORS AND RYANODINE RECEPTORS IN PERIPHERAL COUPLINGS OF BC(3)H1 CELLS", The Journal of cell biology, 137(4), 1997, pp. 859-870

Abstract

Rapid release of calcium from the sarcoplasmic reticulum (SR) of skeletal muscle fibers during excitation-contraction (e-c) coupling is initiated by the interaction of surface membrane calcium channels (dihydropyridine receptors; DHPRs) with the calcium release channels of the SR (ryanodine receptors; RyRs, or feet). We studied the early differentiation of calcium release units, which mediate this interaction, in BC(3)H1 cells. Immunofluorescence labelings of differentiating myocytes with antibodies against alpha(1) and alpha(2) subunits of DHPRs, RyRs,and triadin show that the skeletal isoforms of all four proteins are abundantly expressed upon differentiation, they appear concomitantly, and they are colocalized. The transverse tubular system is poorly organized, and thus clusters of e-c coupling proteins are predominantly located at the cell periphery. Freeze fracture analysis of the surface membrane reveals tetrads of large intramembrane particles, arranged in orderly arrays. These appear concomitantly with arrays of feet (RyRs) and with the appearance of DHPR/RyS clusters, confirming that the fourcomponents of the tetrads correspond to skeletal muscle DHPRs. The arrangement of tetrads and feet in developing junctions indicates that incorporation of DHPRs in junctional domains of the surface membrane proceeds gradually and is highly coordinated with the formation of RyR arrays. Within the arrays, tetrads are positioned at a spacing of twicethe distance between the feet, The incorporation of individual DHPRs into tetrads occurs exclusively at positions corresponding to alternate feet, suggesting that the assembly of RyR arrays not only guides theassembly of tetrads but also determines their characteristic spacing in the junction.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 16/07/20 alle ore 06:15:07