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Titolo:
INHIBITION OF MONKEY SPERM HYALURONIDASE ACTIVITY AND HETEROLOGOUS CUMULUS PENETRATION BY FLAVONOIDS
Autore:
LI MW; YUDIN AI; VANDEVOORT CA; SABEUR K; PRIMAKOFF P; OVERSTREET JW;
Indirizzi:
UNIV CALIF DAVIS,DIV REPROD BIOL & MED,DEPT OBSTET & GYNECOL,SUBER HOUSE DAVIS CA 95616 UNIV CALIF DAVIS,DIV REPROD BIOL & MED,DEPT OBSTET & GYNECOL DAVIS CA95616 UNIV CALIF DAVIS,CALIF REG PRIMATE RES CTR DAVIS CA 95616 UNIV CALIF DAVIS,SCH MED,DEPT CELL BIOL & HUMAN ANAT DAVIS CA 95616
Titolo Testata:
Biology of reproduction
fascicolo: 6, volume: 56, anno: 1997,
pagine: 1383 - 1389
SICI:
0006-3363(1997)56:6<1383:IOMSHA>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
GUINEA-PIG SPERMATOZOA; ZONA-PELLUCIDA; ACROSOME REACTION; GOLDEN-HAMSTER; EXTRACELLULAR-MATRIX; PROTEIN PH-20; OOCYTE; INVITRO; FERTILIZATION; CYNOMOLGUS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
45
Recensione:
Indirizzi per estratti:
Citazione:
M.W. Li et al., "INHIBITION OF MONKEY SPERM HYALURONIDASE ACTIVITY AND HETEROLOGOUS CUMULUS PENETRATION BY FLAVONOIDS", Biology of reproduction, 56(6), 1997, pp. 1383-1389

Abstract

A microplate assay for hyaluronidase and a heterologous cumulus penetration assay were used to determine the effects of four flavonoids (tannic acid, kaempferol, quercetin, and apigenin) on the function of cynomolgus monkey sperm. All four flavonoids inhibited the activity of hyaluronidase extracted from monkey sperm in a concentration-dependent manner over the range of 50-200 mu M Tannic acid and apigenin had lowerinhibitory effects than kaempferol and quercetin. Kaempferol, quercetin, and apigenin at 100 mu M were shown to significantly inhibit monkey sperm penetration into hamster cumulus. There was a significant linear relationship between the capacity of the flavonoids to inhibit monkey sperm hyaluronidase activity and their inhibitory effects on hamster cumulus penetration (r = 0.97). Tannic acid was observed to reduce sperm motility, and it was not used in the cumulus penetration assay. The other three flavonoids tested in the cumulus penetration assay did not affect sperm motility, nor did they induce acrosome reactions. Theresults demonstrate that the flavonoids are useful tools for assessing the involvement of hyaluronidase in the functions of monkey sperm that are involved in fertilization.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 19:39:31