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Titolo:
Glycine immunoreactivity of multipolar neurons in the ventral cochlear nucleus which project to the dorsal cochlear nucleus
Autore:
Doucet, JR; Ross, AT; Gillespie, MB; Ryugo, DK;
Indirizzi:
Johns Hopkins Univ, Sch Med, Dept Otolaryngol Head & Neck Surg, Ctr Hearing Johns Hopkins Univ Baltimore MD USA 21205 l Head & Neck Surg, Ctr Hearing Johns21205ins Univ, Sch Med, Dept Neurosci, Ctr Hearing Sci, Baltimore, MDJohns Hopkins Univ Baltimore MD USA 21205 Ctr Hearing Sci, Baltimore, MD
Titolo Testata:
JOURNAL OF COMPARATIVE NEUROLOGY
fascicolo: 4, volume: 408, anno: 1999,
pagine: 515 - 531
SICI:
0021-9967(19990614)408:4<515:GIOMNI>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
AUDITORY-NERVE FIBERS; DIFFERING SPONTANEOUS RATE; SUPERIOR OLIVARY COMPLEX; GUINEA-PIG; IMMUNOCYTOCHEMICAL LOCALIZATION; INTRACELLULAR-RECORDINGS; INFERIOR COLLICULUS; SLICE PREPARATIONS; SPIKING NEURONS; TRAPEZOID BODY;
Keywords:
auditory system; biotinylated dextran amine; hearing; inhibition;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
65
Recensione:
Indirizzi per estratti:
Indirizzo: Doucet, JR Johns Hopkins Univ, Sch Med, Dept Otolaryngol Head & Neck Surg,Ctr Hearing Johns Hopkins Univ 720 Rutland Ave Baltimore MD USA 21205 aring
Citazione:
J.R. Doucet et al., "Glycine immunoreactivity of multipolar neurons in the ventral cochlear nucleus which project to the dorsal cochlear nucleus", J COMP NEUR, 408(4), 1999, pp. 515-531

Abstract

Certain distinct populations of neurons in the dorsal cochlear nucleus areinhibited by a neural source that is responsive to a wide range of acoustic frequencies. In this study, we examined the glycine immunoreactivity of two types of ventral cochlear nucleus neurons (planar and radiate) in the rat which project to the dorsal cochlear nucleus (DCN) and thus, might be responsible for this inhibition. Previously, we proposed that planar neurons provided a tonotopic and narrowly tuned input to the DCN, whereas radiate neurons provided a broadly tuned input and thus, were strong candidates as the som ce of broadband inhibition (Doucet and Ryugo [1997] J. Comp. Neurol. 385:245-264). We tested this idea by combining retrograde labeling and glycine immunohistochemical protocols. Planar and radiate neurons were first retrogradely labeled by injecting biotinylated dextran amine into a restricted region of the dorsal cochlear nucleus. The labeled cells were visualized using streptavidin conjugated to indocarbocyanine (Cy3), a fluorescent marker. Sections that contained planar or radiate neurons were then processed for glycine immunocytochemistry using diaminobenzidine as the chromogen. Immunostaining of planar neurons was light, comparable to that of excitatory neurons (pyramidal neurons in the DCN), whereas immunostaining of radiate neurons was dark, comparable to that of glycinergic neurons (cartwheel cells in the dorsal cochlear nucleus and principal cells in the medial nucleus ofthe trapezoid body). These results are consistent with the hypothesis thatradiate neurons in the ventral cochlear nucleus subserve the wideband inhibition observed in the dorsal cochlear nucleus. (C) 1999 Wiley-Liss, Inc.

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Documento generato il 19/09/20 alle ore 18:43:12