Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
CC chemokine receptor-3 undergoes prolonged ligand-induced internalization
Autore:
Zimmermann, N; Conkright, JJ; Rothenberg, ME;
Indirizzi:
ChildrensCincinnati,Ctr, Dept Pediat, Div Pulm Med Allergy & Clin Immunol,Childrens Hosp Cincinnati OH USA 45229 v Pulm Med Allergy & Clin Immunol, Childrens Hosp, Med Ctr, Dept Pediat, Div Pulm Biol & Neonatol, Cincinnati, Childrens Hosp Cincinnati OH USA 45229 Pulm Biol & Neonatol, Cincinnati,
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 18, volume: 274, anno: 1999,
pagine: 12611 - 12618
SICI:
0021-9258(19990430)274:18<12611:CCRUPL>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
GUINEA-PIG MODEL; EOTAXIN RECEPTOR; CHEMOATTRACTANT RECEPTORS; EOSINOPHIL RECRUITMENT; CHEMOTACTIC CYTOKINES; ALLERGIC INFLAMMATION; HIV-1 CORECEPTOR; IN-VIVO; EXPRESSION; PROTEIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Rothenberg, ME Childrens3333p, Med Ctr, Dept Pediat, Div Pulm Med Allergy & Clin Immunol, Childrens Hosp 3333 Burnet Ave Cincinnati OH USA 45229 nol,
Citazione:
N. Zimmermann et al., "CC chemokine receptor-3 undergoes prolonged ligand-induced internalization", J BIOL CHEM, 274(18), 1999, pp. 12611-12618

Abstract

CC chemokine receptor-3 (CCR-3) is a major receptor involved in regulatingeosinophil trafficking; therefore, elucidation of ligand-induced CCR-3 events has important implications in understanding the biological and pathological properties of eosinophils. Previous studies have demonstrated that unique receptor events occur in different cell types supporting investigation of CCR-3-mediated events in eosinophilic cells. We now report biochemical characterization of CCR-3 internalization following exposure of eosinophils to CCR-3 ligands, Treatment of freshly isolated human eosinophils with CCR-3 ligands resulted in marked and differential internalization of CCR-3 in adose-dependent manner. Exposure to 100 ng/ml eotaxin reduced surface expression to 43, 43, and 76% at 15 min, 1 h, and 3 h, respectively. RANTES (reduced on activation T cell expressed and secreted) treatment induced more significant and prolonged internalization of CCR-3 than eotaxin; following 100 ng/ml of RANTES, 29, 24, and 47% of the receptor was expressed at 15 min,3 h, and 18 h, respectively. Confocal microscopy demonstrated that receptor modulation involved receptor internalization by an endocytic pathway shared with the transferrin receptor. Receptor internalization was accompanied by partial degradation of CCR-3, and reexpression of CCR-3 was dependent inpart upon de novo protein synthesis. Internalization was not blocked by pretreatment of eosinophils with pertussis toxin. Furthermore, staurosporine did not inhibit internalization although it blocked phorbol la-myristate 13-acetate-induced CCR-3 down-modulation. These results demonstrate that CCR-3 ligands induce differential receptor internalization that is not dependent upon G(i)-protein coupling, calcium transients, or protein kinase C.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/09/20 alle ore 16:47:20