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Titolo:
Genetic analysis and enzyme activity suggest the existence of more than one minimal functional unit capable of synthesizing phosphoribosyl pyrophosphate in Saccharomyces cerevisiae
Autore:
Hernando, Y; Carter, AT; Parr, A; Hove-Jensen, B; Schweizer, M;
Indirizzi:
AFRC,andst Food Res, Dept Genet & Microbiol, Norwich NR4 7UA, Norfolk, Engl AFRC Norwich Norfolk England NR4 7UA biol, Norwich NR4 7UA, Norfolk, Engl Univrkopenhagen, Inst Mol Biol, Ctr Enzyme Res, DK-1307 Copenhagen K, Denma Univ Copenhagen Copenhagen Denmark K me Res, DK-1307 Copenhagen K, Denma
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 18, volume: 274, anno: 1999,
pagine: 12480 - 12487
SICI:
0021-9258(19990430)274:18<12480:GAAEAS>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
SYNTHETASE-ASSOCIATED PROTEIN; COMPLETE CDNA SEQUENCE; BACILLUS-SUBTILIS; ESCHERICHIA-COLI; RAT-LIVER; CATALYTIC SUBUNITS; MOLECULAR-CLONING; PURIFIED ENZYME; PRS; NUCLEOTIDE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
54
Recensione:
Indirizzi per estratti:
Indirizzo: Schweizer, M AFRC,A,nst Food Res, Dept Genet & Microbiol, Norwich Res Pk, Norwich NR4 7U AFRC Norwich Res Pk Norwich Norfolk England NR4 7UA ch NR4 7U
Citazione:
Y. Hernando et al., "Genetic analysis and enzyme activity suggest the existence of more than one minimal functional unit capable of synthesizing phosphoribosyl pyrophosphate in Saccharomyces cerevisiae", J BIOL CHEM, 274(18), 1999, pp. 12480-12487

Abstract

The PRS gene family in Saccharomyces cerevisiae consists of five genes each capable of encoding a 5-phosphoribosyl-1(alpha)-pyrophosphate synthetase polypeptide, To gain insight into the functional organization of this gene family we have constructed a collection of strains containing all possible combinations of disruptions in the five PRS genes. Phenotypically these deletant strains can be classified into three groups: (i) a lethal phenotype that corresponds to strains containing a double disruption in PRS2 and PRS4 in combination with a disruption in either PRS1 or PRS3; simultaneous deletion of PRS1 and PRS5 or PRS3 and PRS5 are also lethal combinations; (ii) a second phenotype that is encountered in strains containing disruptions in PRS and PRS3 together or in combination with any of the other PRS genes manifests itself as a reduction in growth rate, enzyme activity, and nucleotidecontent; (iii) a third phenotype that corresponds to strains that, although affected in their phosphoribosyl pyrophosphate-synthesizing ability, are unimpaired for growth and have nucleotide profiles virtually the same as the mild type. Deletions of PRS2, PRS4, and PRS5 or combinations thereof cause this phenotype, These results suggest that the polypeptides encoded by the members of the PRS gene family may be organized into two functional entities, Evidence that these polypeptides interact with each other in vivo was obtained using the yeast two-hybrid system. Specifically PRS1 and PRS3 polypeptides interact strongly with each other, and there are significant interactions between the PRS5 polypeptide and either the PRS2 or PRS4 polypeptides. These data suggest that yeast phosphoribosyl pyrophosphate synthetase exists in vivo as multimeric complex(es).

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 09:12:41