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Titolo:
Dynamic evaluation of the hepatic uptake and clearance of manganese-based MRI contrast agents: A P-31 NMR study on the isolated and perfused rat liver
Autore:
Colet, JM; Elst, LV; Muller, RN;
Indirizzi:
Univ Mons, Dept Organ Chem, NMR Lab, B-7000 Mons, Belgium Univ Mons MonsBelgium B-7000 Organ Chem, NMR Lab, B-7000 Mons, Belgium
Titolo Testata:
JMRI-JOURNAL OF MAGNETIC RESONANCE IMAGING
fascicolo: 3, volume: 8, anno: 1998,
pagine: 663 - 669
SICI:
1053-1807(199805/06)8:3<663:DEOTHU>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
MN-DPDP; ENHANCEMENT; HEPATOCYTES; METABOLISM; EXPERIENCE; MNDPDP;
Keywords:
MRI contrast agent; liver; MnDPDP; MnCl2; P-31 spectroscopy; perfused organ;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Muller, RN Univ Mons, Dept Organ Chem, NMR Lab, B-7000 Mons, Belgium Univ Mons Mons Belgium B-7000 , NMR Lab, B-7000 Mons, Belgium
Citazione:
J.M. Colet et al., "Dynamic evaluation of the hepatic uptake and clearance of manganese-based MRI contrast agents: A P-31 NMR study on the isolated and perfused rat liver", J MAGN R I, 8(3), 1998, pp. 663-669

Abstract

This spectroscopic study compares the mechanisms of the hepatic uptake of manganese chloride (MnCl2) and manganese dipyridoxyl diphosphate (MnDPDP). Alterations of the phosphorus-31 (P-31)-NMR spectrum of the intracellular adenosine 5'-triphosphate (ATP) are used to monitor the internalization of manganese by the isolated and perfused rat liver. Mn2+ delivered as MnCl2 inthe perfusate rapidly enters the hepatocytes, where it strongly interacts with ATP, inducing a broadening of the 31P lines, The inhibition of the process by nifedipine confirms that manganese ions cross the cellular membraneat least partly through Ca2+ channels, MnDPDP induces weaker but still significant changes of the ATP spectrum. The inability of pyridoxine to compete for the uptake of manganese confirms that the vitamin B-6 carrier is not involved in the internalization process of the paramagnetic complex. Finally, preincubation of MnDPDP with blood does not increase the extent of the dissociation. The alterations of the P-31 spectrum of the liver subsequent to the administration of MnDPDP are attributable to a fraction of free Mn2+ released by the chelate and delivered to the hepatocytes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 18:45:00