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Titolo:
Modulation of lectinophagocytosis of Escherichia coli by variation of pH and temperature
Autore:
Rainho, CS; de Sa, EAC; Gaziri, LCJ; Saridakis, HO; Felipe, I;
Indirizzi:
Univ Estadual Londrina, CCB, Dept Ciencias Patol, BR-86051 Londrina, Brazil Univ Estadual Londrina Londrina Brazil BR-86051 R-86051 Londrina, Brazil Univ Estadual Londrina, Dept Ciencias Fisiol, Londrina, Brazil Univ Estadual Londrina Londrina Brazil iencias Fisiol, Londrina, Brazil Univ Estadual Londrina, Dept Microbiol, Londrina, Brazil Univ Estadual Londrina Londrina Brazil Dept Microbiol, Londrina, Brazil
Titolo Testata:
FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY
fascicolo: 1, volume: 24, anno: 1999,
pagine: 91 - 95
SICI:
0928-8244(199905)24:1<91:MOLOEC>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
MACROPHAGE MANNOSE RECEPTOR; HOST EPITHELIAL-CELLS; PHAGOCYTOSIS; INHIBITION; PIG;
Keywords:
Escherichia coli; lectinophagocytosis; macrophage; polymorphonuclear leukocyte;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
17
Recensione:
Indirizzi per estratti:
Indirizzo: Felipe, I Univ Estadual Londrina, CCB, Dept Ciencias Patol, BR-86051 Londrina, Brazil Univ Estadual Londrina Londrina Brazil BR-86051 ndrina, Brazil
Citazione:
C.S. Rainho et al., "Modulation of lectinophagocytosis of Escherichia coli by variation of pH and temperature", FEMS IM MED, 24(1), 1999, pp. 91-95

Abstract

The effect of variation of pH and temperature on the lectinophagocytosis of enteropathogenic Escherichia coli by polymorphonuclear leukocytes and macrophages elicited by thioglycolate medium was evaluated. The phagocytosis of enteropathogenic E. coli is dependent on pH, being maximal at pH 7.0 and reduced at pH 5.5 or 6.0. Mannan and mannose las representative sugars thatbind to phagocyte lectin receptors), are recognized by mannose receptors and reduced the phagocytic index at pH 7.0 (from 41.6 +/- 8.5 to 17.0 +/- 6.1) and at pH 6.0 (from 24.1 +/- 5.1 to 14.5 +/- 5.0), suggesting that mannose receptors, despite their reduced affinity for ligand at pH 6.0, also participate in phagocytosis of enteropathogenic E. coli. The inhibition of phagocytosis by anti-substance A antibody was also examined at pH 7.0 and at pH 6.0, decreasing (from 41.6+/-8.5 to 21.1 +/- 3.4) and (from 24.1 +/- 5.1 to 12.0 +/- 3.5), respectively. This antibody reduced the phagocytosis of enteropathogenic E. coli in phagocytic assays at 37 or 41 degrees C. These results suggest that the acidic pH decreased the affinity of mannose receptors to ligands on the surface of E. coli and also affected the binding of lectin from E. coli to N-acetylgalactosamine on phagocytes. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

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Documento generato il 12/07/20 alle ore 03:13:03