Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Biosynthesis of HLA-C heavy chains in melanoma cells with multiple defectsin the expression of HLA-A, -B, -C molecules
Autore:
Martayan, A; Fraioli, R; Giorda, E; Setini, A; Ciccarelli, G; Delfino, L; Ferrara, GB; Giacomini, P;
Indirizzi:
Ist Regina Elena CRS, Immunol Lab, I-00158 Rome, Italy Ist Regina Elena CRS Rome Italy I-00158 Immunol Lab, I-00158 Rome, Italy Ist Nazl Ric Canc, Ctr Biotechnol Avanzate, Immunogenet Lab, I-16132 Genoa, Ist Nazl Ric Canc Genoa Italy I-16132 e, Immunogenet Lab, I-16132 Genoa,
Titolo Testata:
BRITISH JOURNAL OF CANCER
fascicolo: 5-6, volume: 80, anno: 1999,
pagine: 639 - 649
SICI:
0007-0920(199905)80:5-6<639:BOHHCI>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
CLASS-I MOLECULES; SURFACE EXPRESSION; MONOCLONAL-ANTIBODIES; ANTIGEN EXPRESSION; BETA-2-MICROGLOBULIN; TAP; BETA(2)-MICROGLOBULIN; PEPTIDE; TAPASIN; INDUCTION;
Keywords:
HLA-C; beta(2)-microglobulin; calnexin; calreticulin; TAP; melanoma;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Giacomini, P Ist Regina Elena CRS, Immunol Lab, Via Messi dOro 156, I-00158 Rome, Italy Ist Regina Elena CRS Via Messi dOro 156 Rome Italy I-00158 y
Citazione:
A. Martayan et al., "Biosynthesis of HLA-C heavy chains in melanoma cells with multiple defectsin the expression of HLA-A, -B, -C molecules", BR J CANC, 80(5-6), 1999, pp. 639-649

Abstract

Recent investigations have shown that malignant transformation may down-regulate the expression of class I HLA molecules, beta(2)-microglobulin (beta(2)m) and members of the antigen-processing machinery. In the present study, we HLA-genotyped and identified at a biochemical level the three (HLA-A25, -B8, -Cw7) class I alleles expressed by the previously described [D'Urso CM et al (1992) J Clin Invest 87: 284-292] beta(2)m-defective human melanoma FO-1 cell line and tested their ability to interact with calnexin, calreticulin and the TAP (transporter associated with antigen processing) complex. Ail these alleles were found to bind calnexin, but not calreticulin or the poorly expressed TAP complex, both in parental and beta(2)m-transfected FO-1 cells, demonstrating a complex defect of class I expression in FO-1 cells. In these conditions, Cw7 heavy chains interacted with calnexin more strongly than A25 and B8, and preferentially accumulated in the endoplasmic reticulum, in both a calnexin-associated and a calnexin-free form. In addition, they could be transported to the cell surface at low levels even in the absence of beta(2)m, without undergoing terminal glycosylation. These results establish a parallel between HLA-C and the murine D-b and L-d molecules which have been found to be surface expressed and functional in beta(2)m-defective cells. They also demonstrate distinctive features of HLA-C molecules. We propose that the accumulation of several assembly intermediates of HLA-C might favour the binding of peptide antigens not readily bound by HLA-Aand -B molecules in neoplastic cells with suboptimal class I expression.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 12:28:54