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Titolo:
The motility of human spermatozoa as influenced by prostasomes at various pH levels
Autore:
Arienti, G; Carlini, E; Nicolucci, A; Cosmi, EV; Santi, F; Palmerini, CA;
Indirizzi:
Ist Biochim & Chim Med, I-06127 Perugia, Italy Ist Biochim & Chim Med Perugia Italy I-06127 Med, I-06127 Perugia, Italy Univ La Sapienza, Dipartimento Ginecol & Ostet, I-00161 Rome, Italy Univ La Sapienza Rome Italy I-00161 Ginecol & Ostet, I-00161 Rome, Italy Dipartimento Biol Cellulare & Mol, I-06127 Perugia, Italy Dipartimento Biol Cellulare & Mol Perugia Italy I-06127 7 Perugia, Italy
Titolo Testata:
BIOLOGY OF THE CELL
fascicolo: 1, volume: 91, anno: 1999,
pagine: 51 - 54
SICI:
0248-4900(199901)91:1<51:TMOHSA>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
SWIM-UP MEDIUM; HUMAN-SPERM; SEMEN; INCLUSION; FUSION;
Keywords:
motility; prostasomes; semen; superimposed image analysis system; spermatozoa;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Arienti, G Ist Biochim & Chim Med, Via Giochetto, I-06127 Perugia, Italy Ist Biochim & Chim Med Via Giochetto Perugia Italy I-06127 aly
Citazione:
G. Arienti et al., "The motility of human spermatozoa as influenced by prostasomes at various pH levels", BIO CELL, 91(1), 1999, pp. 51-54

Abstract

Human semen contains several components among which spermatozoa, membranous vesicles called 'prostasomes', secreted by the prostate gland and unorganized material. Prostasomes possess an unusual lipid composition, contain a number of proteins and small molecules and have been claimed to take a partin the immune response, in seminal fluid liquefaction and in sperm motility. Since sperm may come in contact with an. acidic environment in the vagina, it may be of some interest to know whether prostasomes may affect spermatozoon motility or may protect spermatozoa upon the exposure to an acidic milieu. Human semen was supplied by donors. From whole semen we collected spermatozoa by centrifugation and used the supernatant to prepare prostasomes(centrifugation at 105 000 g for 120 min, followed by purification step onSephadex G 200); spermatozoa were then collected by a swim-up procedure and exposed to an acidic pH medium (from 5 to 7) in the presence or absence of: prostasomes. Spermatozoa motility was subsequently assessed with a superimposed image analysis system (SIAS). Results indicate that the motility ofspermatozoa was affected by the pH value of the medium. Acidic media reduced the percentage of motile cells and decreased the straight line velocity of spermatozoa (VLS). Prostasomes had a protective effect and increased thepercentage of motile cells. However, they did not change the characteristics of motility (curvilinear and straight). Prostasomes may be considered asa system for counteracting the negative effects of acidic pH values that may be present in the vagina after coitus. (C) Elsevier, Paris.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/07/20 alle ore 22:17:19