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Titolo:
Blood-induced joint damage - A human in vitro study
Autore:
Roosendaal, G; Vianen, ME; Marx, JJM; van den Berg, HM; Lafeber, FPJG; Bijlsma, JWJ;
Indirizzi:
Univ Med Ctr Utrecht, Van Creveld Clin, NL-3508 GA Utrecht, Netherlands Univ Med Ctr Utrecht Utrecht Netherlands NL-3508 GA Utrecht, Netherlands
Titolo Testata:
ARTHRITIS AND RHEUMATISM
fascicolo: 5, volume: 42, anno: 1999,
pagine: 1025 - 1032
SICI:
0004-3591(199905)42:5<1025:BJD-AH>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
NECROSIS-FACTOR-ALPHA; HEAT-SHOCK-PROTEIN; ARTICULAR-CARTILAGE; PROTEOGLYCAN SYNTHESIS; HYDROGEN-PEROXIDE; HEMOPHILIC ARTHROPATHY; SYNOVIAL-FLUID; FREE-RADICALS; INHIBITION; PATHOGENESIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Roosendaal, G Univerlandsr Utrecht, Van Creveld Clin, POB 85500, NL-3508 GA Utrecht, Neth Univ Med Ctr Utrecht POB 85500 Utrecht Netherlands NL-3508GA
Citazione:
G. Roosendaal et al., "Blood-induced joint damage - A human in vitro study", ARTH RHEUM, 42(5), 1999, pp. 1025-1032

Abstract

Objective, To investigate mechanisms underlying cartilage damage caused bybrief exposure of cartilage to blood, such as that occurring during intraarticular bleeding. Methods. Human articular cartilage was cultured for 4 days in the presenceof blood (components; 7.5-50% volume/volume). The synthesis of cartilage matrix, as determined by proteoglycan synthesis (incorporation of (SO42-)-S-35), was measured directly after exposure and after a recovery period of 20days, during which the cartilage was cultured in the absence of blood or blood components. The production of the cytokines interleukin-1 (IL-1) and tumor necrosis factor alpha (TNF alpha), which have a destructive effect on cartilage, was determined by enzyme-linked immunosorbent assay, and the viability of chondrocytes was determined by measuring lactate dehydrogenase release and with electron microscopy, The involvement of oxygen metabolites was evaluated by using N-acetylcysteine. Results. Brief exposure to blood resulted in dose-dependent inhibition of proteoglycan synthesis. The combination of mononuclear cells and red blood cells was responsible for this effect. The effect was irreversible, independent of IL-1 and TNF alpha production, and was accompanied by chondrocyte death. These effects were partially prevented by N-acetylcysteine,Conclusion, Brief exposure of cartilage to blood, as occurs after a singleepisode or a limited number of bleeding episodes, results in lasting cartilage damage in vitro, in which cytotoxic oxygen metabolites play a role.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 06:26:27