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Titolo:
Generation of dendritic cells from patients with chronic myelogenous leukemia
Autore:
Heinzinger, M; Waller, CF; von den Berg, A; Rosenstiel, A; Lange, W;
Indirizzi:
Univ Freiburg, Med Ctr, Dept Hematol Oncol, D-79106 Freiburg, Germany UnivFreiburg Freiburg Germany D-79106 Oncol, D-79106 Freiburg, Germany Univ Freiburg, Dept Biol, D-79106 Freiburg, Germany Univ Freiburg Freiburg Germany D-79106 t Biol, D-79106 Freiburg, Germany
Titolo Testata:
ANNALS OF HEMATOLOGY
fascicolo: 4, volume: 78, anno: 1999,
pagine: 181 - 186
SICI:
0939-5555(199904)78:4<181:GODCFP>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHRONIC MYELOCYTIC-LEUKEMIA; PHILADELPHIA-CHROMOSOME; TRANSLOCATION; ABL; MOLECULES; PEPTIDES; IMMUNITY; PROTEIN; MICE;
Keywords:
CML; dendritic cell; FISH; FICTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Lange, W Univburg,burg, Med Ctr, Dept Hematol Oncol, Hugstetter Str 55, D-79106 Frei Univ Freiburg Hugstetter Str 55 Freiburg Germany D-79106 06 Frei
Citazione:
M. Heinzinger et al., "Generation of dendritic cells from patients with chronic myelogenous leukemia", ANN HEMATOL, 78(4), 1999, pp. 181-186

Abstract

Dendritic cells (DCs) are professional antigen-presenting cells (APCs) specialized to internalize, process, and present antigen. They have the capacity to stimulate the primary immune response of resting T-cells. We generated DCs from the adherent cell fraction of peripheral blood, as well as from purified CD34(+) cells from CML patients. Characterizing DCs from ten CML patients by flow cytometry, we found that these cells are highly positive for HLA-DR, CD1a, CD23, and CD80 and negative for CD14, CD15, and CD16. The yield of DCs ranged from 19.5 to 68%. In addition, we used a functional testof FITC-dextran uptake to verify that early DCs take up large particles (0.5-3 mu m) by macropinocytosis while monocytes do not. FITC-dextran uptake was detected by flow cytometry, showing that DCs had accumulated these fluorescent particles. Electron-microscopic analysis showed no major morphological differences between normal and CML-derived DCs. Furthermore, cultured DCs were isolated by FAC sorting for CD1a and HLA-DR expression. In these highly purified cells the Ph chromosome was detected by interphase fluorescence in situ hybridization (FISH) and by fluorescence immunophenotyping and interphase cytogenetics as a tool for the investigation of neoplasms (FICTION); 30-85% of DCs generated were Ph-chromosome positive. It might thereforebe possible not only to prime T-cells with bcr/abl-specific synthetic peptides, but also to stimulate T cells directly with Ph-positive DCs. Use of DCs might serve as a novel therapeutic approach in CML patients, due to their ability to induce highly specific T-cell responses in an autologous system.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 09:37:53