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Titolo:
Neurokinin B- and substance P-like immunoreactivity are co-localized in enteric nerves of rat ileum
Autore:
Yunker, AMR; Krause, JE; Roth, KA;
Indirizzi:
Washington Univ, Sch Med, Dept Pathol, St Louis, MO 63110 USA Washington Univ St Louis MO USA 63110 Dept Pathol, St Louis, MO 63110 USA Washington Univ, Sch Med, Dept Mol Biol & Pharmacol, St Louis, MO 63110 USA Washington Univ St Louis MO USA 63110 & Pharmacol, St Louis, MO 63110 USA Neurogen Corp, Branford, CT 06405 USA Neurogen Corp Branford CT USA 06405Neurogen Corp, Branford, CT 06405 USA
Titolo Testata:
REGULATORY PEPTIDES
fascicolo: 1-2, volume: 80, anno: 1999,
pagine: 67 - 74
SICI:
0167-0115(19990317)80:1-2<67:NBASPI>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
NEUROMEDIN-K; GASTROINTESTINAL-TRACT; MESSENGER-RNAS; MAMMALIAN TACHYKININS; INSITU HYBRIDIZATION; PERIPHERAL-TISSUES; NERVOUS-SYSTEM; SPINAL-CORD; A-LIKE; NEURONS;
Keywords:
tachykinin; submucosal; myenteric; gastrointestinal; intestinal motility;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Yunker, AMR Washington Univ, Sch Med, Dept Pathol, St Louis, MO 63110 USA Washington Univ St Louis MO USA 63110 St Louis, MO 63110 USA
Citazione:
A.M.R. Yunker et al., "Neurokinin B- and substance P-like immunoreactivity are co-localized in enteric nerves of rat ileum", REGUL PEPT, 80(1-2), 1999, pp. 67-74

Abstract

The tachykinins (TKs) substance P (SP), neurokinin A (NKA), and neurokininB (NKB) have conserved C-terminal sequences and mediate similar physiological responses by activating neurokinin receptors found on neural and smoothmuscle cells. Many enteric nerves express preprotachykinin A (PPT A) mRNA and synthesize SP and NKA. However, it is unclear if NKB is synthesized in enteric neurons as many antibodies developed against NKB also recognize other TKs. Therefore, the cellular distribution of NKB-like-immunoreactivity (NKB-ir) in rat ileum was examined using selective antisera raised against either synthetic Cys(10)-NKB or peptide 2 (P2), a non-tachykinergic peptide sequence in NKB precursor protein. NKB-ir and P2-ir had a similar distribution in varicose nerve fibers in submucosal and myenteric ganglia and almostall ganglia contained immunoreactive nerves. Few submucosal or myenteric neuronal somata contained strong immunoreactivity. Preabsorption of NKB or P2 antisera with their respective cognate peptides, but not with other TK peptides, abolished specific immunostaining. Finally, co-localization of NKB-/P2-ir with SP-ir suggested that most NKB-/P2-ir nerve fibers contain SP-ir, but some SP-ir nerves do not contain detectable NKB-/P2-ir. These resultsindicate that PPT B products P2 and NKB are localized in a subpopulation of enteric nerves containing TKs encoded by PPT A. Stimulation of these nerves may release NKB to activate local neurokinin receptors. (C) 1999 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 18:28:20