Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Optical imaging of neural activity in a rat hippocampal slice in an ischemia-like condition
Autore:
Nomura, Y; Kamibayashi, K; Takuma, S; Akaike, T;
Indirizzi:
HokkaidonUniv, Res Inst Elect Sci, Div Biophys, Sapporo, Hokkaido 060, Japa Hokkaido Univ Sapporo Hokkaido Japan 060 hys, Sapporo, Hokkaido 060, Japa Hokkaido Univ, Sch Dent, Dept Oral Physiol, Sapporo, Hokkaido 060, Japan Hokkaido Univ Sapporo Hokkaido Japan 060 ol, Sapporo, Hokkaido 060, Japan
Titolo Testata:
NEUROSCIENCE RESEARCH COMMUNICATIONS
fascicolo: 2, volume: 24, anno: 1999,
pagine: 71 - 80
SICI:
0893-6609(199903/04)24:2<71:OIONAI>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
DEPRIVATION; GLUCOSE; OXYGEN; TRANSMISSION; HYPOXIA; CORTEX; ANOXIA; CELLS;
Keywords:
voltage-sensitive dye; neural activity; optical recording; hippocampus; ischemia;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Nomura, Y HokkaidonUniv, Res Inst Elect Sci, Div Biophys, Sapporo, Hokkaido 060, Japa Hokkaido Univ Sapporo Hokkaido Japan 060 ro, Hokkaido 060, Japa
Citazione:
Y. Nomura et al., "Optical imaging of neural activity in a rat hippocampal slice in an ischemia-like condition", NEUROSC R C, 24(2), 1999, pp. 71-80

Abstract

We visualized neural activity evoked by stimulating Schaffer collaterals in the CA1 region in a rat hippocampal slice preparation, and analyzed the effect of exposure to an ischemia-like condition at room temperature. We recorded also field potentials from the nearby pyramidal cell layer. Optical signals revealed propagation of action potentials in the molecular/radiatum layers in both directions from the stimulated site, followed by activation of pyramidal/oriens layers, and at the later phase spread to more lateral regions. In an ischemia-like condition in which oxygen and glucose were removed from the perfusing fluid, population spikes and/or slow potentials gradually reduced and disappeared, while optical signals remained only at the stimulation locus. When the perfusate solution was switched to the normal fluid within 10 min population spikes and the optical signals restored to thecontrol level after 15-30 min. These observations demonstrated that generation of neural activities in Schaffer collaterals are the most resistive, but that synaptic transmissions are very sensitive to ischemia-like condition.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/20 alle ore 01:00:16