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Titolo:
Expression of dopamine receptors in the subthalamic nucleus of the rat: Characterization using reverse transcriptase polymerase chain reaction and autoradiography
Autore:
Flores, G; Liang, JJ; Sierra, A; Martinez-Fong, D; Quirion, R; Aceves, J; Srivastava, LK;
Indirizzi:
Univ Autonoma Puebla, Inst Fisiol, Puebla 72000, Mexico Univ Autonoma Puebla Puebla Mexico 72000 st Fisiol, Puebla 72000, Mexico McGillnia,v, Dept Psychiat, Douglas Hosp, Res Ctr,McGill Ctr Res Schizophre McGill Univ Verdun PQ Canada H4H 1R3 p, Res Ctr,McGill Ctr Res Schizophre Insteurociencias,l, Ctr Invest & Estudios Avanzados, Dept Fisiol Biofis & N Inst Politecn Nacl Mexico City DF Mexico 07000 os, Dept Fisiol Biofis & N
Titolo Testata:
NEUROSCIENCE
fascicolo: 2, volume: 91, anno: 1999,
pagine: 549 - 556
SICI:
0306-4522(1999)91:2<549:EODRIT>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
NIGRA PARS RETICULATA; SUBSTANTIA-NIGRA; MESSENGER-RNA; BASAL GANGLIA; I-125 IODOSULPRIDE; MOLECULAR-BIOLOGY; BRAIN; BINDING; LESIONS; NEURONS;
Keywords:
basal ganglia; substantia nigra; caudate-putamen; 6-hydroxydopamine; dopamine transporter; Parkinson's disease;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Flores, G UnivoAutonoma Puebla, Inst Fisiol, Apartado Postal 406, Puebla 72000, Mexic Univ Autonoma Puebla Apartado Postal 406 Puebla Mexico 72000 ic
Citazione:
G. Flores et al., "Expression of dopamine receptors in the subthalamic nucleus of the rat: Characterization using reverse transcriptase polymerase chain reaction and autoradiography", NEUROSCIENC, 91(2), 1999, pp. 549-556

Abstract

We analysed the expression of dopamine receptor subtypes in the subthalamic nucleus by means of reverse transcriptase-polymerase chain reaction. We also studied, using autoradiography, all pharmacologically characterized dopamine receptors in four subregions of the subthalamic nucleus. For comparison, dopamine receptor subtypes were also evaluated in brain regions where they are more abundant and well characterized. The radioligands used were: [H-3]SCH-23390, [H-3]emonapride and [H-3]2-dipropylamino-7-hydroxy-1,2,3,4-tetrahydronaphthalene for dopamine D-1, D-2 and D-3 receptors, respectively;and [H-3]YM-09151-2 in the presence of raclopride for dopamine D-4 receptors. Finally, we also evaluated the effect of unilateral 6-hydroxydopamine injection into the medial forebrain bundle on dopamine receptor levels expressed in the ipsilateral subthalamic nucleus, The lesion was estimated by decrease in the binding of [H-3]WIN-35428, a specific dopamine transporter label. D-1, D-2 and D-3 receptor messenger RNAs and binding sites were present in the subthalamic nucleus, but no messenger RNA for D-1 receptors was found, although specific binding sites for these receptors were observed. As compared to the intact side, the 6-hydroxydopamine lesion did not change D-1 receptors, increased D-2 receptors, and decreased D-3 receptors and the dopamine transporter. The results suggest that postsynaptic D-1, D-2 or D-3 receptors can mediate the effect of dopamine on subthalamic nucleus neuronal activity. D-4 receptors would mediate exclusively presynaptic effects. These results reinforce the idea that dopamine receptors in the subthalamic nucleus may play an important role in the physiology of the basal gangliaand in the pathophysiology of Parkinson's disease. (C) 1999 IBRO. Published by Elsevier Science Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 14:39:43