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Titolo:
Generation of monoclonal antibodies to native human immunodeficiency virustype 1 envelope glycoprotein by immunization of mice with naked RNA
Autore:
Giraud, A; Ataman-Onal, Y; Battail, N; Piga, N; Brand, D; Mandrand, B; Verrier, B;
Indirizzi:
Ecoleol,rmale Super Lyon, CNRS, UMR103, Unite Mixte BioMerieux,Lab Retrovir Ecole Normale Super Lyon Lyon France 07 te Mixte BioMerieux,Lab Retrovir BioMerieux, Dept R&D, Marcy Letoile, France BioMerieux Marcy Letoile France erieux, Dept R&D, Marcy Letoile, France Univ Tours, CNRS, EP 117, Virol Lab, Tours, France Univ Tours Tours France v Tours, CNRS, EP 117, Virol Lab, Tours, France
Titolo Testata:
JOURNAL OF VIROLOGICAL METHODS
fascicolo: 1, volume: 79, anno: 1999,
pagine: 75 - 84
SICI:
0166-0934(199904)79:1<75:GOMATN>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
VACCINIA VIRUS; GENETIC IMMUNIZATION; IMMUNE-RESPONSES; DNA VACCINATION; GP120; EXPRESSION; PROTEIN; RECOMBINANTS; PARTICLES; CULTURE;
Keywords:
monoclonal antibody; immunization; human immunodeficiency virus type 1; envelope glycoprotein; Semliki Forest virus vector; naked RNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Verrier, B Ecoleol,rmale Super Lyon, CNRS, UMR103, Unite Mixte BioMerieux,Lab Retrovir Ecole Normale Super Lyon 46 Allee Italie Lyon France 07 trovir
Citazione:
A. Giraud et al., "Generation of monoclonal antibodies to native human immunodeficiency virustype 1 envelope glycoprotein by immunization of mice with naked RNA", J VIROL MET, 79(1), 1999, pp. 75-84

Abstract

The Semliki Forest virus (SFV) vector system is a new approach for in vivoexpression of heterologous proteins and can also be used to generate specific immune responses in animal models. HIV-1 envelope glycoprotein producedusing the SFV expression system is correctly folded, cleaved, transported to the cell surface and exhibits functional activity. We evaluated a recombinant Semliki Forest virus naked RNA-based immunization protocol for generation of monoclonal antibodies against the HIV-I envelope glycoprotein. In vitro-transcribed RNA encoding for the SFV replicase complex and Env proteinof HIV-1 (HXB2 strain) was injected intramuscularly to mice. This approachelicited an Env-specific antibody response in four mice out of five and a monoclonal antibody, 12H2, directed against gp41 was produced. Our results show that recombinant SFV RNA immunization can potentially be used as a quick and direct method to produce monoclonal antibodies, with the particular advantage that vectored RNA, rather than purified antigen, delivers a complex oligomer produced correctly. (C) 1999 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 18:22:03