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Titolo:
Cyclin T1 domains involved in complex formation with Tat and TAR RNA are critical for tat-activation
Autore:
Ivanov, D; Kwak, YT; Nee, E; Guo, J; Garcia-Martinez, LF; Gaynor, RB;
Indirizzi:
Univ,Texas, SW Med Ctr, Harold Simmons Canc Ctr, Dept Med,Div Hematol Oncol Univ Texas Dallas TX USA 75235 mmons Canc Ctr, Dept Med,Div Hematol Oncol
Titolo Testata:
JOURNAL OF MOLECULAR BIOLOGY
fascicolo: 1, volume: 288, anno: 1999,
pagine: 41 - 56
SICI:
0022-2836(19990423)288:1<41:CTDIIC>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
IMMUNODEFICIENCY-VIRUS TYPE-1; C-TERMINAL DOMAIN; TRANSCRIPTION-ELONGATION COMPLEX; PROTEINS SPECIFICALLY ASSOCIATE; FACTOR P-TEFB; POLYMERASE-II; HIV-1 TAT; IN-VITRO; TRANS-ACTIVATION; HUMAN CHROMOSOME-12;
Keywords:
HIV; Tat; cyclin T; CDK9; P-TEFb;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
65
Recensione:
Indirizzi per estratti:
Indirizzo: Gaynor, RB Univ,Texas, SW Med Ctr, Harold Simmons Canc Ctr, Dept Med,Div Hematol Oncol Univ Texas 5323 Harry Hines Blvd Dallas TX USA 75235 atol Oncol
Citazione:
D. Ivanov et al., "Cyclin T1 domains involved in complex formation with Tat and TAR RNA are critical for tat-activation", J MOL BIOL, 288(1), 1999, pp. 41-56

Abstract

Tat activates transcription from the human immunodeficiency virus type 1 (HIV-1) long terminal repeat (LTR) by increasing the processivity of RNA polymerase II. Recently, it has been demonstrated that the cellular kinase CDK9 and its binding partner cyclin T1 are involved in regulating transcriptional elongation and tat-activation. Cyclin T1, CDK9 and Tat bind as a complex to elements in TAR RNA that are required for tat-activation. Here, we used cyclin T1 mutants to define domains in this protein that bind to both CDK9 and Tat and are involved in stimulating tat-activation. The region of cyclin T1 extending from amino acid residues 1 to 263 is necessary for complexformation with Tat bound to TAR RNA and for stimulation of tat-activation in murine cells that are normally poorly responsive to the actions of Tat. In contrast, a smaller region of cyclin T1 was required to bind to CDK9 andstimulate its kinase activity. Recombinant cyclin T1 and CDK9 stimulated both basal and tat-induced in vitro transcriptional elongation from the HIV-1 LTR. The effects of Tat on transcriptional elongation may be mediated by its ability to increase CDK9 phosphorylation of the RNA polymerase II C-terminal domain. These results demonstrate that cyclin T1 interactions with Tat and TAR RNA are critical for activation of HIV-1 gene expression. (C) 1999 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 06:06:19