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Titolo:
Complete two-dimensional separation for analysis of acidic compounds in plasma using column-switching reversed-phase liquid chromatography
Autore:
Okuda, T; Nakagawa, Y; Motohashi, M;
Indirizzi:
Takeda Chem Ind Ltd, Preformulat Res Lab, Yodogawa Ku, Osaka 5328686, Japan Takeda Chem Ind Ltd Osaka Japan 5328686 odogawa Ku, Osaka 5328686, Japan Takeda Chem Ind Ltd, Drug Anal & Pharmacokinet Res Labs, Yodogawa Ku, Osaka Takeda Chem Ind Ltd Osaka Japan 5328686 net Res Labs, Yodogawa Ku, Osaka
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1-2, volume: 726, anno: 1999,
pagine: 225 - 236
SICI:
1387-2273(19990416)726:1-2<225:CTSFAO>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
ION-PAIR CHROMATOGRAPHY; ULTRAVIOLET DETECTION; HUMAN-SERUM; ELECTROCHEMICAL DETECTION; DIRECT INJECTION; EXTRACTION; METABOLITE; URINE; HPLC;
Keywords:
two-dimensional separation; acidic compounds; zidovudine-beta-D-glucuronide; probenecid;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Okuda, T Takeda Chem Ind Ltd, Preformulat Res Lab, Yodogawa Ku, Juso Honmachi, Osaka Takeda Chem Ind Ltd Juso Honmachi Osaka Japan 5328686 chi, Osaka
Citazione:
T. Okuda et al., "Complete two-dimensional separation for analysis of acidic compounds in plasma using column-switching reversed-phase liquid chromatography", J CHROMAT B, 726(1-2), 1999, pp. 225-236

Abstract

A complete two-dimensional separation technique for the determination of acidic compounds in plasma was developed by using column-switching reversed-phase liquid chromatography. This technique was based on solute peak enrichment at the top of the second column during heart-cutting and an ion-pair chromatographic separation in the second column using tetrabutylammonium ion, where different separation modes in the first and second columns and solute peak enrichment at the top of the second column during heart-cutting were achieved coincidentally. Retention behaviors of two solutes, zidovudine-beta-D-glucuonide (AZT-beta-D-Gluc) and probenecid, in the first and second column and solute peak enrichment at the top of the second column were investigated for establishment of the system. Different retention behaviors of the solutes in the first and second column, which were evaluated by changesin capacity factor versus acetonitrile concentration in the mobile phases,and peak enrichment could be accomplished by using ion-pair chromatographyin the second column. System suitability was confirmed by assessing the number of theoretical plates (N) of the second column for the solutes after heart-cutting. The N values in the second column after column switching werealmost same as those in the case that the solutes were directly injected onto the second column. These results indicate that complete two-dimensionalseparation should be achieved by using this system. Furthermore, this technique was applied to method development for the determination of AZT-beta-D-Gluc and probenecid in rat plasma. The peaks of each analyte in the plasmaextract obtained by deproteinization were well separated from those of endogenous substances, and easy determination of the analytes could be accomplished at the ng/ml level only by changing the acetonitrile concentration inthe mobile phases. (C) 1999 Elsevier Science B.V. All rights reserved.

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Documento generato il 25/01/20 alle ore 15:56:52