Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
A novel glycosylphosphatidylinositol in African trypanosomes - A possible catabolic intermediate
Autore:
Milne, KG; Ferguson, MAJ; Englund, PT;
Indirizzi:
Univ Dundee, Dept Biochem, Dundee DD1 5EH, Scotland Univ Dundee Dundee Scotland DD1 5EH pt Biochem, Dundee DD1 5EH, Scotland Johns Hopkins Univ, Sch Med, Dept Biol Chem, Baltimore, MD 21205 USA JohnsHopkins Univ Baltimore MD USA 21205 l Chem, Baltimore, MD 21205 USA
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 3, volume: 274, anno: 1999,
pagine: 1465 - 1471
SICI:
0021-9258(19990115)274:3<1465:ANGIAT>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
VARIANT SURFACE GLYCOPROTEIN; PHOSPHATIDYLINOSITOL MEMBRANE ANCHORS; DE-N-ACETYLASE; PHOSPHOLIPASE-C; GLYCOLIPID PRECURSORS; RESISTANT GLYCOLIPIDS; MYRISTATE EXCHANGE; INOSITOL-ACYLATION; BIOSYNTHESIS; BRUCEI;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Milne, KG Univ Dundee, Dept Biochem, Wellcome Trust Bldg, Dundee DD1 5EH, Scotland Univ Dundee Wellcome Trust Bldg Dundee Scotland DD1 5EH cotland
Citazione:
K.G. Milne et al., "A novel glycosylphosphatidylinositol in African trypanosomes - A possible catabolic intermediate", J BIOL CHEM, 274(3), 1999, pp. 1465-1471

Abstract

The major glycosylphosphatidylinositols (GPIs) in African trypanosomes areglycolipid A, the precursor of the variant surface glycoprotein membrane anchor, and glycolipid C, a species identical to glycolipid A except that itcontains an acylated inositol. Both glycolipids A and C contain dimyristoyl glycerol and are efficiently labeled with [H-3]myristate in a cell free system. We now report a novel GPI known as lipid X. This GPI is radio-labeled strongly with [H-3]palmitate (and very poorly with [H-3]myristate or [H-3]stearate) in digitonin-permeabilized cells. The structure of lipid X is Man(1)GlcNAc-(2-O-palmitoyl)-D-myo-inositol-1-HPO4-3(lyso-palmitoylglycerol). Metabolically, lipid X exists as an intermediate, and can be detected onlyunder conditions in which its formation is stimulated (e.g. by EDTA) or its breakdown is inhibited (e.g. by Co2+). Lipid X has not been observed previously because these conditions do not support GPI biosynthesis We speculate that lipid X is an intermediate in the catabolism of conventional trypanosome GPIs possibly deriving from breakdown of glycolipid C.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 10:38:05