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Titolo:
Inhibition of serum diamine oxidase discloses a constitutive putrescine release from cultured vascular smooth muscle cells
Autore:
Nilsson, BO; Kockum, I; Rosengren, E;
Indirizzi:
Univ Lund, Dept Physiol & Neurosci, S-22362 Lund, Sweden Univ Lund Lund Sweden S-22362 t Physiol & Neurosci, S-22362 Lund, Sweden
Titolo Testata:
INFLAMMATION RESEARCH
fascicolo: 4, volume: 48, anno: 1999,
pagine: 176 - 180
SICI:
1023-3830(199904)48:4<176:IOSDOD>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECTIFIER K+ CHANNELS; RAT SMALL-INTESTINE; MAMMALIAN-CELLS; POLYAMINES; BINDING; SPERMINE; CALCIUM; EXPORT; BLOOD;
Keywords:
aminoguanidine; diamine oxidase; putrescine release;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Nilsson, BO Univ Lund, Dept Physiol & Neurosci, Solvegatan 19, S-22362 Lund, Sweden Univ Lund Solvegatan 19 Lund Sweden S-22362 2362 Lund, Sweden
Citazione:
B.O. Nilsson et al., "Inhibition of serum diamine oxidase discloses a constitutive putrescine release from cultured vascular smooth muscle cells", INFLAMM RES, 48(4), 1999, pp. 176-180

Abstract

Objective: To determine if putrescine and the higher polyamines spermidineand spermine are released from cultured vascular smooth muscle cells. Material: Vascular smooth muscle cell line A7r5. Treatment: Cells were treated with aminoguanidine (10 or 100 mu M) for 1 to 24 h with or without fetal calf serum (10%) present in the culture medium. Methods: Cellular and medium concentrations of polyamines were determined by liquid chromatography. Total cellular protein was determined by the Bradford procedure. Student's two-tailed t-test was used for statistical calculations. Results: A constitutive release of putrescine was disclosed within 5 h if serum diamine oxidase was inhibited by 10 mu M aminoguanidine. The release was linear with time for 24 h and specific for putrescine in the sense thatthe higher polyamines spermidine and spermine, despite similar cellular concentrations, were not released. Similar amounts of putrescine were released from the smooth muscle cells whether or not culture medium contained serum. Cells, that had been cultured in medium from which fetal calf serum had been omitted for last 48 h, contained less putrescine, spermidine and protein than those that persisted in medium that contained serum. Conclusion: A constitutive putrescine release from vascular smooth muscle cells is disclosed in the presence of aminoguanidine.

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Documento generato il 05/12/20 alle ore 01:25:06