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Titolo:
atSRp30, one of two SF2/ASF-like proteins from Arabidopsis thaliana, regulates splicing of specific plant genes
Autore:
Lopato, S; Kalyna, M; Dorner, S; Kobayashi, R; Krainer, AR; Barta, A;
Indirizzi:
Univ Vienna, Vienna Bioctr, Inst Biochem, A-1030 Vienna, Austria Univ Vienna Vienna Austria A-1030 , Inst Biochem, A-1030 Vienna, Austria Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA Cold Spring Harbor Lab Cold Spring Harbor NY USA 11724 rbor, NY 11724 USA
Titolo Testata:
GENES & DEVELOPMENT
fascicolo: 8, volume: 13, anno: 1999,
pagine: 987 - 1001
SICI:
0890-9369(19990415)13:8<987:AOOTSP>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
PRE-MESSENGER-RNA; SMALL NUCLEAR RIBONUCLEOPROTEIN; SR PROTEINS; PREMESSENGER RNA; CONSERVED FAMILY; SITE SELECTION; 5'-SPLICE-SITE RECOGNITION; SUBNUCLEAR LOCALIZATION; DISTINCT FUNCTIONS; ENHANCER COMPLEX;
Keywords:
SR proteins; alternative splicing; pre-mRNA processing; plant development; overexpression; phenotype;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
83
Recensione:
Indirizzi per estratti:
Indirizzo: Barta, A Univ Vienna, Vienna Bioctr, Inst Biochem, A-1030 Vienna, Austria Univ Vienna Vienna Austria A-1030 ochem, A-1030 Vienna, Austria
Citazione:
S. Lopato et al., "atSRp30, one of two SF2/ASF-like proteins from Arabidopsis thaliana, regulates splicing of specific plant genes", GENE DEV, 13(8), 1999, pp. 987-1001

Abstract

SR proteins are nuclear phosphoproteins with a characteristic Ser/Arg-richdomain and one or two RNA recognition motifs. They are highly conserved inanimals and plants and play important roles in spliceosome assembly and alternative splicing regulation. We have now isolated and partially sequenceda plant protein, which crossreacts with antibodies to human SR proteins. The sequence of the corresponding cDNA and genomic clones from Arabidopsis revealed a protein, atSRp3a, with strong similarity to the human SR protein SF2/ASF and to atSRp34/SR1, a previously identified SR protein, indicating that plants possess two SP2/ASF-like proteins, atSRp30 expresses alternatively spliced mRNA isoforms that are expressed differentially in various organs and during development. Overexpression of atSRp30 via a strong constitutive promoter resulted in changes in alternative splicing of several endogenous plant genes, including atSRp30 itself. Interestingly, atSRp30 overexpression resulted in a pronounced down-regulation of endogenous mRNA encoding full-length atSRp34/SR1 protein. Transgenic plants overexpressing atSRp30 showed morphological and developmental changes affecting mostly developmental phase transitions. atSRp30- and atSRp34/SR1-promoter-GUS constructs exhibited complementary expression patterns during early seedling development and root formation, with overlapping expression in floral tissues. The results of the structural and expression analyses of both genes suggest that atSRp34/SR1 acts as a general splicing factor, whereas atSRp30 functions as a specific splicing modulator.

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Documento generato il 02/12/20 alle ore 14:29:31