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Titolo:
Transcript splicing is essential for functional Histoplasma capsulatum URA5 expression
Autore:
Retallack, DM; Woods, JP;
Indirizzi:
UnivAWisconsin, Sch Med, Dept Med Microbiol & Immunol, Madison, WI 53706 US Univ Wisconsin Madison WI USA 53706 obiol & Immunol, Madison, WI 53706 US
Titolo Testata:
GENE
fascicolo: 2, volume: 230, anno: 1999,
pagine: 181 - 185
SICI:
0378-1119(19990416)230:2<181:TSIEFF>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
YEAST; MACROPHAGES; MUTATIONS; SPLICEOSOME; ALVEOLAR; AIDS; GENE;
Keywords:
filamentous fungi; mRNA processing; uracil auxotrophy; yeast;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Woods, JP UnivAWisconsin, Sch Med, Dept Med Microbiol & Immunol, Madison, WI 53706 US Univ Wisconsin Madison WI USA 53706 munol, Madison, WI 53706 US
Citazione:
D.M. Retallack e J.P. Woods, "Transcript splicing is essential for functional Histoplasma capsulatum URA5 expression", GENE, 230(2), 1999, pp. 181-185

Abstract

The isolation of auxotrophic markers is important for molecular genetic studies of the dimorphic fungus Histoplasma capsulatum. We have isolated a UV-induced mutant of H. capsulatum, resulting in nonreverting uracil auxotrophy due to a mutation in the URA5 gene. In this study, we show that this mutation is a GG to TA conversion bordering the 5' donor splice site of intron2. The mutation results in the lack of splicing of intron 2 from the URA5 transcript, and subsequently premature termination of the peptide. This study is the first showing that consensus Group II intron sequences are both utilized and essential for functional expression of a gene in H. capsulatum. (C) 1999 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 14:44:45