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Titolo:
Induction of p21(CIP1/Waf1) and activation of p34(cdc2) involved in retinoic acid-induced apoptosis in human hepatoma Hep3B cells
Autore:
Hsu, SL; Chen, MC; Chou, YH; Hwang, GY; Yin, SC;
Indirizzi:
Taichung Vet Gen Hosp, Dept Educ & Res, Taichung 40705, Taiwan Taichung Vet Gen Hosp Taichung Taiwan 40705 Res, Taichung 40705, Taiwan Tunghai Univ, Dept Biol, Taichung 40704, Taiwan Tunghai Univ Taichung Taiwan 40704 iv, Dept Biol, Taichung 40704, Taiwan
Titolo Testata:
EXPERIMENTAL CELL RESEARCH
fascicolo: 1, volume: 248, anno: 1999,
pagine: 87 - 96
SICI:
0014-4827(19990410)248:1<87:IOPAAO>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYCLIN-DEPENDENT KINASE-1; P53-MEDIATED G(1) ARREST; C-MYC; PROMYELOCYTIC LEUKEMIA; CANCER CHEMOPREVENTION; NEUROBLASTOMA-CELLS; GROWTH ARREST; DEATH; P53; PROTEIN;
Keywords:
apopotsis; retinoic acid; p34(cdc2); p21(CIP2/Waf1); Bax;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Hsu, SL Taichung40705,en Hosp, Dept Educ & Res, 160,Sect 3,Chung Gang Rd, Taichung Taichung Vet Gen Hosp 160,Sect 3,Chung Gang Rd Taichung Taiwan 40705
Citazione:
S.L. Hsu et al., "Induction of p21(CIP1/Waf1) and activation of p34(cdc2) involved in retinoic acid-induced apoptosis in human hepatoma Hep3B cells", EXP CELL RE, 248(1), 1999, pp. 87-96

Abstract

The biological activity of retinoic acid (RA) was examined in human hepatoma Hep3B cells, Under serum-deprived conditions, RA induced S/M-phase elevation and mitotic index increase within 24 h, followed by apoptosis, This RA-induced apoptosis was accompanied by p53-independent up-regulation of endogenous p21(CIPI/Waf1) proteins, as well as activation of p34(cdc2) kinase, and increase of Rb2 protein level and phosphorylation pattern. In addition,RA had no effect on the levels of Bcl-X-L; Bcl-X-S; cyclins A, B, D1, D3, or E; or Rbl expression but markedly downmodulated Cdk2 kinase activity andreduced Cdk4 expression, RA also slightly delayed p27(Kip1) expression. Olomoucine, a potent p34(cdc2) and Cdk2 inhibitor, effectively blocked RA-mediated p34(cdc2) kinase activation and prevented RA-induced apoptosis. Furthermore, antisense oligonucleotide complementary to p21(CIP2/Waf1) and p34(cdc2) mRNA significantly rescued RA-induced apoptosis. Our data indicate that p21(CIP2/Waf1) overexpression may not be the only regulatory factor necessary for RA-induced apoptosis in human hepatoma Hep3B cells. RA treatment leads to Rb2 hyperphosphorylation, and p34(cdc2) kinase activation is coincident with an aberrant mitotic progression, followed by appearance of abnormal nucleus. This aberrant cell cycle progression appeared requisite for RA-induced cell death. These findings suggest that inappropriate regulation ofthe cell cycle regulators p21(CIP2/Waf1) and p34(cdc2) is coupled with induction of Bar and involved in cell death with apoptosis when Hep3B cells are exposed to RA. (C) 1999 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/20 alle ore 07:23:56