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Titolo:
pQuattro vectors allow one-step multigene metabolic engineering and auto-selection of quattrocistronic artificial mammalian operons
Autore:
Fussenegger, M; Moser, S; Bailey, JE;
Indirizzi:
SwissrlandInst Technol, ETH Zurich, Inst Biotechnol, CH-8093 Zurich, Switze Swiss Fed Inst Technol Zurich Switzerland CH-8093 CH-8093 Zurich, Switze
Titolo Testata:
CYTOTECHNOLOGY
fascicolo: 1-3, volume: 28, anno: 1998,
pagine: 229 - 235
SICI:
0920-9069(1998)28:1-3<229:PVAOMM>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
HAMSTER OVARY CELLS; GENE-EXPRESSION; PRODUCTIVITY; PROTEIN; FAMILY;
Keywords:
CITE; EMCV; GFP; IRES; picornavirus;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
17
Recensione:
Indirizzi per estratti:
Indirizzo: Bailey, JE SwissrlandInst Technol, ETH Zurich, Inst Biotechnol, CH-8093 Zurich, Switze Swiss Fed Inst Technol Zurich Switzerland CH-8093 ich, Switze
Citazione:
M. Fussenegger et al., "pQuattro vectors allow one-step multigene metabolic engineering and auto-selection of quattrocistronic artificial mammalian operons", CYTOTECHNOL, 28(1-3), 1998, pp. 229-235

Abstract

Based on internal ribosomal entry sites (IRES) of picornaviral origin we constructed a novel family of mammalian expression vectors, pQuattro vectorscontain quattrocistronic artificial eukaryotic operons which link, in a single transcript, the simultaneous and coordinated as well as adjustable expression of up to three independent genes of interest to a terminal neomycin(neo) resistance marker. Due to the strict genetic linkage of the transgenes and the terminal selection marker, this genetic configuration enables, by the selection on neomycin, multigene metabolic engineering of mammalian cells in a single step (one-step metabolic engineering). Furthermore, selection on the terminal cistron of multicistronic expression units enforces cocistronic expression of all upstream encoded genes and maximises genetic integrity of the eukaryotic operon in stable mammalian cell lines, since clones harbouring damaged multicistronic expression units become neomycin-sensitive and are automatically counterselected (auto-selection). The modular set-up and the abundance of restriction sites in pQuattro vectors facilitate the movement of individual genes between multicistronic expression vectors and guarantees high compatibility with genetic elements of a wide variety ofexisting mammalian expression vectors.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 19:22:20