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Titolo:
Regulated multicistronic expression technology for mammalian metabolic engineering
Autore:
Fussenegger, M; Moser, S; Bailey, JE;
Indirizzi:
SwissrlandInst Technol, ETH Zurich, Inst Biotechnol, CH-8093 Zurich, Switze Swiss Fed Inst Technol Zurich Switzerland CH-8093 CH-8093 Zurich, Switze
Titolo Testata:
CYTOTECHNOLOGY
fascicolo: 1-3, volume: 28, anno: 1998,
pagine: 111 - 125
SICI:
0920-9069(1998)28:1-3<111:RMETFM>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
INDUCIBLE GENE-EXPRESSION; RIBOSOMAL ENTRY SITE; DICISTRONIC MESSENGER-RNA; HAMSTER OVARY CELLS; CAP-INDEPENDENT TRANSLATION; GREEN FLUORESCENT PROTEIN; 5' NONCODING REGION; INTERNAL INITIATION; RETROVIRAL VECTORS; ENCEPHALOMYOCARDITIS VIRUS;
Keywords:
autoregulation; cell-cycle engineering; eukaryotic operon; IRES; multigene engineering; picornavirus; pTRIDENT; regulated expression;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
124
Recensione:
Indirizzi per estratti:
Indirizzo: Bailey, JE SwissrlandInst Technol, ETH Zurich, Inst Biotechnol, CH-8093 Zurich, Switze Swiss Fed Inst Technol Zurich Switzerland CH-8093 ich, Switze
Citazione:
M. Fussenegger et al., "Regulated multicistronic expression technology for mammalian metabolic engineering", CYTOTECHNOL, 28(1-3), 1998, pp. 111-125

Abstract

Contemporary basic research is rapidly revealing increasingly complex molecular regulatory networks which are often interconnected via key signal integrators. These connections among regulatory and catalytic networks often frustrate bioengineers as promising metabolic engineering strategies are bypassed by compensatory metabolic responses or cause unexpected, undesired outcomes such as apoptosis, product protein degradation or inappropriate post-translational modification. Therefore, for metabolic engineering to achieve greater success in mammalian cell culture processes and to become important for future applications such as gene therapy and tissue engineering, this technology must be enhanced to allow simultaneous, in cases conditional, reshaping of metabolic pathways to access difficult-to-attain cell states. Recent advances in this new territory of multigene metabolic engineering are intimately linked to the development of multicistronic expression technology which allows the simultaneous, and in some cases, regulated expression of several genes in mammalian cells. Here we review recent achievements in multicistronic expression technology in view of multigene metabolic engineering.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 21:05:59