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Titolo:
Manipulation of cellular excitability by cell fusion - Effects of rapid introduction of transient outward K+ current on the guinea pig action potential
Autore:
Hoppe, UC; Johns, DC; Marban, E; ORourke, B;
Indirizzi:
Johns205pkins Univ, Sect Mol & Cellular Cardiol, Dept Med, Baltimore, MD 21 Johns Hopkins Univ Baltimore MD USA 21205 iol, Dept Med, Baltimore, MD 21
Titolo Testata:
CIRCULATION RESEARCH
fascicolo: 8, volume: 84, anno: 1999,
pagine: 964 - 972
SICI:
0009-7330(19990430)84:8<964:MOCEBC>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN VENTRICULAR MYOCYTES; HEART-FAILURE; ELECTROPHYSIOLOGICAL PROPERTIES; REGIONAL DIFFERENCES; POTASSIUM CURRENT; CARDIAC MYOCYTES; CURRENT-DENSITY; MUSCLE; CHANNEL; HETEROGENEITY;
Keywords:
transient outward current; action potential; cell fusion; repolarization;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Johns, DC Johns0Hopkins Univ, Sect Mol & Cellular Cardiol, Dept Med, 844 Ross Bldg,72 Johns Hopkins Univ 844 Ross Bldg,720 Rutland Ave Baltimore MD USA 21205
Citazione:
U.C. Hoppe et al., "Manipulation of cellular excitability by cell fusion - Effects of rapid introduction of transient outward K+ current on the guinea pig action potential", CIRCUL RES, 84(8), 1999, pp. 964-972

Abstract

To investigate the still-undetermined role of :the Ca2+-independent transient outward current (I-to1) on repolarization of the cardiac action potential, we used cell fusion to introduce I-to1 into guinea pig cardiomyocytes, which normally lack this current.:This technique enables the rapid deliveryof premade functional ion channels to cardiomyocytes within hours of isolation, thus eliminating the action potential-alterations that complicate prolonged cell culture. Chinese hamster ovary(CHO) cells stably expressing Kv4.3:(CHO-Kv4.3)- were loaded with a fluorescent dye and fused to guinea pig cardiomyocytes using polyethylene glycol, As controls; nontransfected CHO cells were fused using the same protocol. Myocytes fused with CHO-Kv4.3, cells exhibited a:robust I-tol ( 16.5+/-2.6 pA/pF at +40 mV; 37 degrees C; n=19), whereas controls had none. I-to1 accelerated the early repolarization velocity (r= -0.68; 3 ms after the overshoot) and progressively suppressed the voltage of the-plateau phase (r= -0.90) with increasing I-to1 density. Reduction of the action potential duration to 50% repolarization (r= -0.76) and to 90% repolarization (r= -0.65) also correlated well with I-to1 density. Thus, I-to1 exerted a significant effect on the early repolarization phase and abbreviated action potential duration. Cell fusion is a valuable andgeneralizable technique to introduce preformed membrane proteins into native cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 06:15:18