Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Androgen exposure increases human monocyte adhesion to vascular endothelium and endothelial cell expression of vascular cell adhesion molecule-1
Autore:
McCrohon, JA; Jessup, W; Handelsman, DJ; Celermajer, DS;
Indirizzi:
Royal Prince Alfred Hosp, Dept Cardiol, Sydney, NSW 2050, Australia Royal Prince Alfred Hosp Sydney NSW Australia 2050 y, NSW 2050, Australia Royal Prince Alfred Hosp, Heart Res Inst, Sydney, NSW 2050, Australia Royal Prince Alfred Hosp Sydney NSW Australia 2050 y, NSW 2050, Australia Royal Prince Alfred Hosp, Dept Androl, Sydney, NSW 2050, Australia Royal Prince Alfred Hosp Sydney NSW Australia 2050 y, NSW 2050, Australia Univ Sydney, Dept Med, Sydney, NSW 2006, Australia Univ Sydney Sydney NSWAustralia 2006 pt Med, Sydney, NSW 2006, Australia
Titolo Testata:
CIRCULATION
fascicolo: 17, volume: 99, anno: 1999,
pagine: 2317 - 2322
SICI:
0009-7322(19990504)99:17<2317:AEIHMA>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
CORONARY-ARTERY DISEASE; LOW-DENSITY-LIPOPROTEIN; ISCHEMIC-HEART-DISEASE; CARDIOVASCULAR-DISEASE; MYOCARDIAL-INFARCTION; DEHYDROEPIANDROSTERONE SULFATE; HUMAN ATHEROSCLEROSIS; SEX-HORMONES; POSTMENOPAUSAL ESTROGEN; E-SELECTIN;
Keywords:
hormones; atherosclerosis; cell adhesion molecules;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: McCrohon, JA Royaltraliae Alfred Hosp, Dept Cardiol, Missenden Rd, Sydney,NSW 2050, Aus Royal Prince Alfred Hosp Missenden Rd Sydney NSW Australia 2050
Citazione:
J.A. McCrohon et al., "Androgen exposure increases human monocyte adhesion to vascular endothelium and endothelial cell expression of vascular cell adhesion molecule-1", CIRCULATION, 99(17), 1999, pp. 2317-2322

Abstract

Background-Male sex is an independent risk factor for coronary artery disease. Owing to the importance of monocyte adhesion to endothelial cells in the development of atherosclerosis, we hypothesized that androgens might promote this process. We therefore studied the effects of the nonaromatizable androgen dihydrotestosterone (DHT) on human monocyte adhesion to human endothelial cells and on endothelial cell-surface expression of adhesion molecules. Methods and Results-Human umbilical vein endothelial cells (HUVECs) were grown to confluence in media supplemented with postmenopausal female serum, then exposed for 48 hours to either DHT (40 and 400 nmol/L), with or without the androgen receptor blocker hydroxyflutamide (HF) (4 mu mol/L); HF alone; or vehicle control (ethanol 0.1%). Human monocytes obtained by elutriation were incubated for 1 hour with the HUVECs at 37 degrees C, and adhesion was measured by light microscopy. Compared with vehicle control, monocyte adhesion was increased in the androgen-treated HUVECs in a dose-dependent manner (116 +/- 6% and 128 +/- 3% for DHT 40 and 400 nmol/L respectively; P <0.001). HF blocked this increase (P greater than or equal to 0.3 compared with control). Surface expression of endothelial cell adhesion molecules was measured by ELISA and revealed an increased expression of vascular cell adhesion molecule-1 (VCAM-1) in the DHT-treated HUVECs (125 +/- 5% versus 100 +/- 4% in controls; P = 0.002), an effect also antagonized by HF (P greater than or equal to 0.3 compared with controls). Furthermore, the DHT-related increase in adhesion was completely blocked by coincubation with anti-VCAM-1 antibody. Comparable results were obtained in arterial endothelial cells and in endothelium stimulated with the cytokine tumor necrosis factor-alpha,Conclusions-Androgen exposure is associated with increased human monocyte adhesion to endothelial cells, a proatherogenic effect mediated at least inpart by an increased endothelial cell-surface expression of VCAM-1.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/07/20 alle ore 21:43:26