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Titolo:
Aldehyde dismutase activity of yeast alcohol dehydrogenase
Autore:
Trivic, S; Leskova, V; Winston, GW;
Indirizzi:
Univ Novi Sad, Fac Technol, YU-21000 Novi Sad, Yugoslavia Univ Novi Sad Novi Sad Yugoslavia YU-21000 YU-21000 Novi Sad, Yugoslavia Fac Sci Novi Sad, YU-21000 Novi Sad, Yugoslavia Fac Sci Novi Sad Novi Sad Yugoslavia YU-21000 21000 Novi Sad, Yugoslavia N Carolina State Univ, Raleigh, NC 27695 USA N Carolina State Univ Raleigh NC USA 27695 te Univ, Raleigh, NC 27695 USA
Titolo Testata:
BIOTECHNOLOGY LETTERS
fascicolo: 3, volume: 21, anno: 1999,
pagine: 231 - 234
SICI:
0141-5492(199903)21:3<231:ADAOYA>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
PRIMARY ALIPHATIC-ALCOHOLS; MECHANISM; PH; OXIDATION; REDUCTION; KINETICS; BINDING;
Keywords:
alcohol dehydrogenase; aldehyde dismutase; enzyme kinetics; Saccharomyces cerevisiae; yeast;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Leskova, V Univlavia Sad, Fac Technol, Bulevar Cara Lazara 1, YU-21000 Novi Sad, Yugos Univ Novi Sad Bulevar Cara Lazara 1 Novi Sad Yugoslavia YU-21000
Citazione:
S. Trivic et al., "Aldehyde dismutase activity of yeast alcohol dehydrogenase", BIOTECH LET, 21(3), 1999, pp. 231-234

Abstract

Yeast alcohol dehydrogenase (EC 1.1.1.1) is able to catalyze the oxidationof acetaldehyde by NAD(+) with a concomitant formation of ethanol, at pH 8.8 and pH 7.1; the stoichiometry of aldehyde oxidation vs, ethanol formation is 2:1. This enzymatic reaction obeys the Michaelis-Menten kinetics and was characterized by a high K-M for acetaldehyde (68 mM) and a low k(cat) (2.3 s(-1)), at pH 8.8, 22 degrees C. There is no visible burst of NADH during the reaction, from pH 7.1-10.1. Therefore, we have concluded that the enzyme catalyzes an apparent dismutation of two molecules of acetaldehyde intoa molecule of acetic acid and a molecule of ethanol.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 19:02:30