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Titolo:
Lattice simulations of protein crystal formation
Autore:
Kierzek, AM; Pokarowski, P; Zielenkiewicz, P;
Indirizzi:
Polish Acad Sci, Inst Biochem & Biophys, PL-02106 Warsaw, Poland Polish Acad Sci Warsaw Poland PL-02106 Biophys, PL-02106 Warsaw, Poland Polish Acad Sci, Inst Math, PL-00950 Warsaw, Poland Polish Acad Sci Warsaw Poland PL-00950 nst Math, PL-00950 Warsaw, Poland
Titolo Testata:
BIOPHYSICAL CHEMISTRY
fascicolo: 2-3, volume: 77, anno: 1999,
pagine: 123 - 137
SICI:
0301-4622(19990329)77:2-3<123:LSOPCF>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
LYSOZYME SOLUTIONS; CRYSTALLIZATION; GROWTH; NUCLEATION; SCATTERING; KINETICS; RECOGNITION; ASSOCIATION; CONTACTS;
Keywords:
protein crystallisation; lattice simulations; lysozyme;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Zielenkiewicz, P Polisholand Sci, Inst Biochem & Biophys, Pawinskiego 5A, PL-02106 Warsaw, P Polish Acad Sci Pawinskiego 5A Warsaw Poland PL-02106 P
Citazione:
A.M. Kierzek et al., "Lattice simulations of protein crystal formation", BIOPHYS CH, 77(2-3), 1999, pp. 123-137

Abstract

A new algorithm is presented for the lattice simulation of protein crystalgrowth. The algorithm allows the calculation of the size distribution of microcrystals in the volume and timescale of experiments and within the framework of the previously-published microscopic model [A.M. Kierzek, W.M. Wolf, P. Zielenkiewicz, Biophys. J. 73 (1997) 571-580]. Simulations for the tetragonal lysozyme crystal show that there are two critical sizes in the development of ordered phase. The first one corresponds to the size of the smallest stable complex which, in the case of the tetragonal lysozyme crystal,is the particular tetramer. In a volume of 5 mu l the tetramer appears in the millisecond timescale. The second critical radius of approximately 100 monomers is only reached by a few of all the smallest stable complexes formed in the solution. The model predicts that out of 10(7) tetramers which appear in solution, only eight reach the size of 100 monomers within 8 h. After exceeding the second critical radius the microcrystals grow to the size of 10(4) monomers in the minute timescale and are thus assumed to quickly lead to macroscopic crystals. The predicted number of crystals formed during8 h of nucleation is in qualitative agreement with arrested nucleation experiments. (C) 1999 Elsevier Science B.V. All rights reserved.

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Documento generato il 30/09/20 alle ore 09:39:10