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Titolo:
Association of polo-like kinase with alpha-, beta- and gamma-tubulins in astable complex
Autore:
Feng, Y; Hodge, DR; Palmieri, G; Chase, DL; Longo, DL; Ferris, DK;
Indirizzi:
NCI,,Frederick Canc Res & Dev Ctr, Biol Mechanisms Sect, Lab Leukocyte Biol NCI Frederick MD USA 21702 Ctr, Biol Mechanisms Sect, Lab Leukocyte Biol NIA, Baltimore, MD 21224 USA NIA Baltimore MD USA 21224NIA, Baltimore, MD 21224 USA NCI,02rederick Canc Res & Dev Ctr, Sci Applicat Int Corp, Frederick, MD 217 NCI Frederick MD USA 21702 Ctr, Sci Applicat Int Corp, Frederick, MD 217
Titolo Testata:
BIOCHEMICAL JOURNAL
, volume: 339, anno: 1999,
parte:, 2
pagine: 435 - 442
SICI:
0264-6021(19990415)339:<435:AOPKWA>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
PEPTIDYL-PROLYL ISOMERASE; XENOPUS EGG EXTRACTS; PROTEIN-KINASE; SACCHAROMYCES-CEREVISIAE; SERINE/THREONINE KINASE; DROSOPHILA POLO; GENE; PLK1; IDENTIFICATION; SPINDLE;
Keywords:
cell-cycle regulation; kinases; spindle formation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Ferris, DK NCI,,Frederick Canc Res & Dev Ctr, Biol Mechanisms Sect, Lab Leukocyte Biol NCI Frederick MD USA 21702 Mechanisms Sect, Lab Leukocyte Biol
Citazione:
Y. Feng et al., "Association of polo-like kinase with alpha-, beta- and gamma-tubulins in astable complex", BIOCHEM J, 339, 1999, pp. 435-442

Abstract

The polo-like kinase (Plk) family has been shown to have an important rolein the regulation of the cell-division cycle, especially in organization of the spindle structure, in species from fungi to humans. Recent reports have demonstrated that in mammalian cells Plk is associated with components of the anaphase-promoting complex and a peptidyl-prolyl isomerase, Pin1. To characterize a putative Plk-containing complex, we fractionated mitotic cell lysates on a gel-filtration column. The Plk complex was eluted from the column at molecular sizes ranging from 669 to 2500 kDa in the presence of detergent and high concentrations of salt, Specific associations of Plk with alpha-, beta- and gamma-tubulins in both interphase and mitotic cells were shown by reciprocal immunoprecipitations and immunoblottings and were independent of the microtubule polymerization state, whereas binding assays in vitro indicated that Plk interacts with alpha- and beta-tubulins directly. In addition, mitotic Plk was able to phosphorylate associated tubulins in vitro. Finally, we show that the kinase domain of the Plk molecule is both required and sufficient for its binding to tubulins in vivo. The specific interaction between Plk and tubulins might provide a molecular basis for the physiological functions of Plk in regulating the cell cycle, particularly inestablishing the normal bipolar spindle.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 09:48:58