Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
NOS inhibitors decrease hypoxia-induced ATP reductions in respiring cerebrocortical slices
Autore:
Litt, L; Espanol, MT; Hasegawa, K; Chang, LH; Gregory, GA; James, TL; Beal, MF; Chan, PH;
Indirizzi:
Univ Calif San Francisco, Dept Anesthesia, San Francisco, CA 94143 USA Univ Calif San Francisco San Francisco CA USA 94143 ancisco, CA 94143 USA Univ Calif San Francisco, Dept Radiol, San Francisco, CA 94143 USA Univ Calif San Francisco San Francisco CA USA 94143 ancisco, CA 94143 USA Univ Calif San Francisco, Dept Pharmaceut Chem, San Francisco, CA 94143 USA Univ Calif San Francisco San Francisco CA USA 94143 ancisco, CA 94143 USA Univ Calif San Francisco, Dept Pediat, San Francisco, CA 94143 USA Univ Calif San Francisco San Francisco CA USA 94143 ancisco, CA 94143 USA Univ Calif San Francisco, Cardiovasc Res Inst, San Francisco, CA 94143 USAUniv Calif San Francisco San Francisco CA USA 94143 ancisco, CA 94143 USA Stanford Univ, Dept Neurol, Stanford, CA 94305 USA Stanford Univ StanfordCA USA 94305 , Dept Neurol, Stanford, CA 94305 USA Cornell Univ, Coll Med, Dept Neurol, New York, NY 10021 USA Cornell Univ New York NY USA 10021 d, Dept Neurol, New York, NY 10021 USA Stanford Univ, Dept Neurosurg, Stanford, CA 94305 USA Stanford Univ Stanford CA USA 94305 ept Neurosurg, Stanford, CA 94305 USA
Titolo Testata:
ANESTHESIOLOGY
fascicolo: 5, volume: 90, anno: 1999,
pagine: 1392 - 1401
SICI:
0003-3022(199905)90:5<1392:NIDHAR>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
NITRIC-OXIDE SYNTHASE; FOCAL CEREBRAL-ISCHEMIA; HSP70 MESSENGER-RNA; NEONATAL RAT; BRAIN-SLICES; PROTEIN INHIBITOR; KYNURENIC ACID; C-FOS; INJURY; EXPRESSION;
Keywords:
anoxia; bioenergetics; brain metabolism; nitric oxide; nuclear magnetic resonance;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Litt, L Univcisco, San Francisco, Dept Anesthesia, Box 0648,521 Parnassus,San Fran Univ Calif San Francisco Box 0648,521 Parnassus San Francisco CA USA 94143
Citazione:
L. Litt et al., "NOS inhibitors decrease hypoxia-induced ATP reductions in respiring cerebrocortical slices", ANESTHESIOL, 90(5), 1999, pp. 1392-1401

Abstract

Background: Excess neuronal nitric oxide (NO) production might cause adenosine triphosphate loss and cellular damage in hypoxic brain parenchyma, (31)p nuclear magnetic resonance spectroscopy was used to study hypoxic intracellular responses in perfused respiring cerebrocortical slices, in which NOscavenging by hemoglobin is absent, during NO synthase blockade and NO augmentation. Methods: Adenosine triphosphate concentrations were monitored at 4.7 Teslain respiring slices before, during, and after 60 min of hypoxia (oxygen tension < 5 mmHg), Slices were not treated or were pretreated with 27 mu M L-nitroarginine methyl ester (L-NAME), 27 mu M 7-nitroindozole (7-NI), or 27 mu M L-nitroarginine. Nitrotyrosine:tyrosine ratios of slice extracts were measured using high-performance liquid chromatography. Cresyl violet-stained sections (2 mu m) from random slices n ere examined histologically. Results: After 60 min of hypoxia, adenosine triphosphate decreased to lessthan or equal to 3, less than or equal to 3, 65 +/- 6, and 25 +/- 4% of control in slices that were untreated or treated with L-nitroarginine, L-NAME, and 7-NI, respectively. After 120 min of hyperoxic recovery, adenosine triphosphate levels returned to control values in slices pretreated with L-NAME and 7-NI, but to only 30% of control in untreated or nitroarginine-treated slices, Nitric oxide donors administered during posthypoxic recovery partially antagonized the adenosine triphosphate recovery found with L-NAME and 7-NI, Nitric oxide synthase activity in slice homogenates, assayed via conversion of L-arginine to citrulline, was less than or equal to 2% of control after all inhibitory treatments. The nitro-tyrosine:tyrosine ratio increased by 52% in slices treated with 7-NI and by 200-300% in all other groups. Pretreatment with L-NAME and 7-NI reduced histologic evidence of cell swelling. Conclusion: Neuronal NO is associated with rapid adenosine triphosphate reductions and peroxynitrite formation in acutely hypoxic cerebrocortical slices.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/04/20 alle ore 22:37:42