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Titolo:
Evaluation of fine root length and diameter measurements obtained using RHIZO image analysis
Autore:
Bauhus, J; Messier, C;
Indirizzi:
Minist Forests, Res Branch, Forest Biol Sect, Victoria, BC V8W 9C2, CanadaMinist Forests Victoria BC Canada V8W 9C2 t, Victoria, BC V8W 9C2, Canada Australian Natl Univ, Dept Forestry, Canberra, ACT 0200, Australia Australian Natl Univ Canberra ACT Australia 0200 rra, ACT 0200, Australia
Titolo Testata:
AGRONOMY JOURNAL
fascicolo: 1, volume: 91, anno: 1999,
pagine: 142 - 147
SICI:
0002-1962(199901/02)91:1<142:EOFRLA>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
COMPUTER; SCANNER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
13
Recensione:
Indirizzi per estratti:
Indirizzo: Messier, C Univ Quebec, Dept Sci Biol, GREF, CP 8888, Montreal, PQ H3C 3P8, Canada Univ Quebec CP 8888 Montreal PQ Canada H3C 3P8 H3C 3P8, Canada
Citazione:
J. Bauhus e C. Messier, "Evaluation of fine root length and diameter measurements obtained using RHIZO image analysis", AGRON J, 91(1), 1999, pp. 142-147

Abstract

Image analysis systems facilitate rapid measurement of root length and diameter, but their accuracy is not easily determined. The objective of this study was to develop a set of simple experiments for evaluating the accuracyof fine root measurements obtained using image analysis, Using the system RHIZO (trademark of Regent Instruments, Quebec), we tested the accuracy of (i) length measurements made over a range of root lengths per unit area, (ii) average diameter measurements and length per diameter distributions in string, wire, and fine root samples of varying diameter, and (iii) diameter measurements on short segments of diagonally oriented objects. Our results suggest that preliminary testing of image analysis systems is absolutely necessary for producing reliable root measurements. Total length was accurately determined for typically encountered length per unit areas of < 1.5 cm cm(-1). For samples with higher values, however, the method underestimated total length by >5%. It is therefore recommended that users of image analysis systems determine this maximum length per unit area for accurate determinations of total root length, In samples that contained different string diameters, the total sample length and average string diameter could accurately be measured. However, the length per diameter class was underestimated by>20% when the string diameter was less than one pixel smaller than the upper limit of the diameter class. Adjustment of diameter intervals and increasing the scanner resolution are required to reduce this underestimation. Both the length and the angle of the short segments analyzed were found to influence diameter measurements. Similar sets of experiments are proposed fora rigorous evaluation of the performance of other image analysis systems on root measurements.

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Documento generato il 29/11/20 alle ore 00:33:09