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Titolo:
Relationship between tissue growth, CO2 level and tendril formation duringin vitro culture of grape (Vitis vinifera L.)
Autore:
Thomas, P;
Indirizzi:
Indian Inst Hort Res, Div Biotechnol, Bangalore 560089, Karnataka, India Indian Inst Hort Res Bangalore Karnataka India 560089 9, Karnataka, India
Titolo Testata:
VITIS
fascicolo: 1, volume: 38, anno: 1999,
pagine: 25 - 29
SICI:
0042-7500(199903)38:1<25:RBTGCL>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-VITRO; MICROPROPAGATED GRAPES; GAS-EXCHANGE; PLANTLETS; INVITRO; ACCLIMATIZATION; INDUCTION;
Keywords:
CO2; light; grapes; in vitro; micropropagation; photosynthesis; rejuvenation; tendril;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Thomas, P Indian Inst Hort Res, Div Biotechnol, Hessaraghatta Lake, Bangalore 560089, Indian Inst Hort Res Hessaraghatta Lake Bangalore Karnataka India 560089
Citazione:
P. Thomas, "Relationship between tissue growth, CO2 level and tendril formation duringin vitro culture of grape (Vitis vinifera L.)", VITIS, 38(1), 1999, pp. 25-29

Abstract

In vitro cultures of grapevine cultivar Arka Neelamani growing in MURASHIGE and SKOOG (1962) medium containing 3% sucrose and incubated under 16 h light (30-10 mu mol.m(-2)s(-1)) per day showed high CO2 levels (600-11900 ppm) at the end of the dark period and a significant decline during the light period (330-980 ppm) indicating photosynthetic activity and photomixotrophic growth characteristic. The CO2 level at the end of a 8-h-dark period was shown to be related to the total tissue weight (wt) per culture Vessel while that during the light period was governed by the root to shoot fresh wt ratio suggesting a notable contribution by roots and inadequate light supplyfor full CO2 utilization. Plant growth was improved by higher light intensities (40-50 mu mol degrees m(-2 degrees)s(-1)). Tendril production, a characteristic of adult plants, was common during the first year of culture butshowed a gradual decline by the second and third year suggesting a gradualrejuvenation effect in vitro. Transformation of tendril, an organ homologous to inflorescence, to leafy structure in some plants and better rooting with more subcultures in vitro further supported the rejuvenation hypothesis. In general, tendril production was mon common in vessels with higher CO2 levels. Plants established ex vitro invariably showed tendril production within 1-3 months indicating that the in vitro rejuvenation effect was not transmitted to the field.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 14:32:27