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Titolo:
Adenovirus E4 open reading frame 4-induced dephosphorylation inhibits E1A activation of the E2 promoter and E2F-1-mediated transactivation independently of the retinoblastoma tumor suppressor protein
Autore:
Mannervik, M; Fan, S; Strom, AC; Helin, K; Akusjarvi, G;
Indirizzi:
Uppsala Univ, BMC, Dept Med Biochem & Microbiol, S-75123 Uppsala, Sweden Uppsala Univ Uppsala Sweden S-75123 & Microbiol, S-75123 Uppsala, Sweden European Inst Oncol, Dept Expt Oncol, I-20141 Milan, Italy European Inst Oncol Milan Italy I-20141 Expt Oncol, I-20141 Milan, Italy
Titolo Testata:
VIROLOGY
fascicolo: 2, volume: 256, anno: 1999,
pagine: 313 - 321
SICI:
0042-6822(19990410)256:2<313:AEORF4>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELLULAR TRANSCRIPTION FACTOR; UBIQUITIN-PROTEASOME PATHWAY; DNA-BINDING ACTIVITY; EARLY REGION-4; GENE-PRODUCT; FAMILY PROTEINS; FACTORS E2F-1; CYCLE CONTROL; DEGRADATION; SEQUENCES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Akusjarvi, G UppsalaSweden BMC, Dept Med Biochem & Microbiol, Box 582, S-75123 Uppsala, Uppsala Univ Box 582 Uppsala Sweden S-75123 S-75123 Uppsala,
Citazione:
M. Mannervik et al., "Adenovirus E4 open reading frame 4-induced dephosphorylation inhibits E1A activation of the E2 promoter and E2F-1-mediated transactivation independently of the retinoblastoma tumor suppressor protein", VIROLOGY, 256(2), 1999, pp. 313-321

Abstract

Previous studies have shown that the cell cycle-regulated E2F transcription factor is subjected to both positive and negative control by phosphorylation. Here we show that in transient transfection experiments, adenovirus EIA activation or the viral E2 promoter is abrogated by coexpression of the viral E4 open reading frame 4 (E4-ORF4) protein. This effect does not to require the retinoblastoma protein that previously has been shown to regulate E2F activity. The inhibitory activity of E4-ORF4 appears to be specific because E4-ORF4 had little effect on, for example, E4-ORF6/7 transactivation of the E2 promoter. We further show that the repressive effect of E4-ORF4 onE2 transcription works mainly through the E2F DNA-binding sites in the 52 promoter. In agreement with this, we find that E4-ORF4 inhibits E2F1/DP-1-mediated transactivation. We also show that E4-ORF4 inhibits E2 mRNA expression during virus growth. E4-ORF4 has previously been shown to bind to and activate the cellular protein phosphatase 2A. The inhibitory effect of E4-ORF4 was relieved by okadaic acid, which inhibits protein phosphatase 2A activity, suggesting that E4-ORF4 represses E2 transcription by inducing transcription factor dephosphorylation. Interestingly, E4-ORF4 did not inhibit the transactivation capacity of a Gal4-E2F hybrid protein. Instead, E4-ORF4 expression appears to result in reduced stability of E2F/DNA complexes. (C) Press Academic Press.

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Documento generato il 09/07/20 alle ore 20:58:47