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Titolo:
Study of the presence of Campylobacter jejuni and C-coli in sand samples from four Swiss chicken farms
Autore:
Studer, E; Luthy, J; Hubner, P;
Indirizzi:
Univ Bern, Dept Chem & Biochem, Food Chem Lab, CH-3012 Bern, Switzerland Univ Bern Bern Switzerland CH-3012 d Chem Lab, CH-3012 Bern, Switzerland
Titolo Testata:
RESEARCH IN MICROBIOLOGY
fascicolo: 3, volume: 150, anno: 1999,
pagine: 213 - 219
SICI:
0923-2508(199904)150:3<213:SOTPOC>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; BROILER FLOCKS; PCR; EPIDEMIOLOGY; COLONIZATION; ENTERITIS; SURVIVAL; PRODUCTS; POULTRY; WASHES;
Keywords:
Campylobacter jejuni; Campylobacter coli; chicken farming; epidemiology;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Studer, E Univitzerlandpt Chem & Biochem, Food Chem Lab, Freiestr 3, CH-3012 Bern, Sw Univ Bern Freiestr 3 Bern Switzerland CH-3012 CH-3012 Bern, Sw
Citazione:
E. Studer et al., "Study of the presence of Campylobacter jejuni and C-coli in sand samples from four Swiss chicken farms", RES MICROB, 150(3), 1999, pp. 213-219

Abstract

Chicken farms are frequently infected with Campylobacter jejuni and Camplyobacter coli. The objective of the present study was to investigate environmental samples from chicken farms for the presence of C. jejuni and C, coli. Every week between July and November 1997, three sand samples from the runs of four chicken farms were analyzed by culture and directly by polymerase chain reaction (PCR). These two detection methods were compared to each other. A total of 231 samples were tested. Eleven samples (4%) were found tocontain Campylobacter cells by culture, whereas 157 samples (68%) were positive by PCR. All samples which were positive by culture were also positiveby PCR. All direct PCR products were further typed by restriction fragmentlength polymorphism (RFLP). Three different RFLP types and mixtures of these types were observed. Direct PCR products of one chicken farm were further typed by direct sequencing and two temporally separated sequence types could be distinguished. Campylobacter strains isolated by culture were also typed by RFLP and direct sequencing revealing close accordance with the corresponding direct PCR products. (C) Elsevier, Paris.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 10:17:22