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Titolo:
Expression of gonadotropin-releasing hormone receptor mRNA in the rat ventral prostate and Dunning R3327 PAP adenocarcinoma before and after castration
Autore:
Tieva, A; Wikstrom, P; Olofsson, JI; Bergh, A; Damber, JE;
Indirizzi:
Umea Univ, Dept Urol & Androl, S-90187 Umea, Sweden Umea Univ Umea Sweden S-90187 , Dept Urol & Androl, S-90187 Umea, Sweden Umea Univ, Dept Gynecol & Obstet, S-90187 Umea, Sweden Umea Univ Umea Sweden S-90187 ept Gynecol & Obstet, S-90187 Umea, Sweden Umea Univ, Dept Pathol, S-90187 Umea, Sweden Umea Univ Umea Sweden S-90187 ea Univ, Dept Pathol, S-90187 Umea, Sweden
Titolo Testata:
PROSTATE
fascicolo: 2, volume: 39, anno: 1999,
pagine: 101 - 107
SICI:
0270-4137(19990501)39:2<101:EOGHRM>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
HIGH-AFFINITY BINDING; HUMAN OVARIAN-CANCER; CELL-LINE LNCAP; ANTAGONIST SB-75; HUMAN-BREAST; ENDOMETRIAL CANCER; ESTROGEN-TREATMENT; GENE-EXPRESSION; MESSENGER-RNA; GROWTH-FACTOR;
Keywords:
GnRH-R; ventral prostate; prostatic adenocarcinoma; castration; competitive reverse transcription-polymerase chain reaction;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Damber, JE Umea Univ, Dept Urol & Androl, S-90187 Umea, Sweden Umea Univ Umea Sweden S-90187 & Androl, S-90187 Umea, Sweden
Citazione:
A. Tieva et al., "Expression of gonadotropin-releasing hormone receptor mRNA in the rat ventral prostate and Dunning R3327 PAP adenocarcinoma before and after castration", PROSTATE, 39(2), 1999, pp. 101-107

Abstract

BACKGROUND. Continuous administration of gonadotropin-releasing hormone (GnRH) agonists in prostate cancer patients results in involution of the tumors due to suppression of androgen production. In addition to the effect of GnRH at the hypothalamic-pituitary level, experiments in vitro on breast, ovary, and prostatic cells have shown an inhibition of cell proliferation, indicating the presence of local GnRH receptors (GnRH-R). The aim of the present study was to investigate the expression of GnRH-R mRNA in the normal rat ventral prostate (VP) and Dunning R3327 PAP adenocarcinoma and to evaluate the effects of castration on receptor mRNA expression. METHODS. RNA was prepared from ovaries, pituitaries, VP, and Dunning tumors from both intact and castrated animals. GnRH-R mRNA levels were quantified by a competitive reverse transcription-polymerase chain reaction (RT-PCR)method. RESULTS. GnRH-R mRNA was detected in normal VP and Dunning tumors. Normal VP showed lower amounts of GnRH-R mRNA compared to Dunning tumors. An elevation of mRNA expression was observed 7 days after castration in Dunning tumors. CONCLUSIONS. GnRH-R mRNA was found in both VP and Dunning tumors, indicating the presence of a local GnRH system. Normal VP showed lower amounts of GnRH-R mRNA when compared to malignant tissues. GnRH-R mRNA levels were elevated in Dunning tumors following castration. (C) 1999 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 00:01:28