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Titolo:
p53 mutations in tumors derived from irradiated human thyroid epithelial cells
Autore:
Gamble, SC; Cook, MC; Riches, AC; Herceg, Z; Bryant, PE; Arrand, JE;
Indirizzi:
Brunel Univ, Dept Biol & Biochem, Uxbridge UB8 3PH, Middx, England Brunel Univ Uxbridge Middx England UB8 3PH bridge UB8 3PH, Middx, England Univ St Andrews, Sch Biomed Sci, St Andrews KY16 9TS, Fife, Scotland Univ St Andrews St Andrews Fife Scotland KY16 9TS Y16 9TS, Fife, Scotland
Titolo Testata:
MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS
fascicolo: 2, volume: 425, anno: 1999,
pagine: 231 - 238
SICI:
1386-1964(19990406)425:2<231:PMITDF>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
RADIATION-INDUCED TRANSFORMATION; IN-VITRO; SUPPRESSOR GENE; LUNG-CANCER; DNA-DAMAGE; CARCINOMAS; EXPOSURE; SINGLE; SURVIVAL; OCCUR;
Keywords:
p53; ionizing radiation; thyroid tumor;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Gamble, SC Brunel Univ, Dept Biol & Biochem, Uxbridge UB8 3PH, Middx, England Brunel Univ Uxbridge Middx England UB8 3PH 3PH, Middx, England
Citazione:
S.C. Gamble et al., "p53 mutations in tumors derived from irradiated human thyroid epithelial cells", MUT RES-F M, 425(2), 1999, pp. 231-238

Abstract

A non-tumorigenic human thyroid epithelial cell line (HTori-3) has been transformed into tumorigenic cells by exposure in vitro to alpha particles orgamma-radiation. These transformants were tumorigenic in athymic nude miceand tumors were transplantable into other nude mice. To further characterize processes involved in neoplastic progression, the tumor cell lines derived from these radiation-induced primary tumors were screened for mutations in the p53 tumor suppressor gene. p53 mutation was detected by single-strand conformation polymorphism (SSCP) analysis of exons 5 to 8 inclusive. Mutations detected by SSCP analysis were confirmed by sequencing. Mutations were detected in all four exons analysed, although there was no correlation between dose, LET or mutation position or frequency. Mutations in p53 exons 6and 7 have been reported in the childhood papillary thyroid carcinomas in Belarus presumably as a result of radioiodine fall-out. Similarly here, p53mutations are induced experimentally during the development of human thyroid tumors generated by irradiation of a human thyroid epithelial cell line in vitro, (C) 1999 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 09:58:27