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Titolo:
Cotranscription of the rpl5-rps14-cob gene cluster in pea mitochondria
Autore:
Hoffmann, M; Dombrowski, S; Guha, C; Binder, S;
Indirizzi:
Univ Ulm, Allgemeine Bot, D-89069 Ulm, Germany Univ Ulm Ulm Germany D-89069 v Ulm, Allgemeine Bot, D-89069 Ulm, Germany
Titolo Testata:
MOLECULAR AND GENERAL GENETICS
fascicolo: 3, volume: 261, anno: 1999,
pagine: 537 - 545
SICI:
0026-8925(199904)261:3<537:COTRGC>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSCRIPTION INITIATION SITES; RIBOSOMAL-PROTEIN S10; MESSENGER-RNA; ARABIDOPSIS MITOCHONDRIA; PLANT-MITOCHONDRIA; SOLANUM-TUBEROSUM; APOCYTOCHROME-B; IN-VITRO; GENOME; SEQUENCE;
Keywords:
plant mitochondria; polycistronic RNA; RNA processing; mitochondrial promoters;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Binder, S Univ Ulm, Allgemeine Bot, Albert-Einstein-Allee 11, D-89069 Ulm,Germany Univ Ulm Albert-Einstein-Allee 11 Ulm Germany D-89069 , Germany
Citazione:
M. Hoffmann et al., "Cotranscription of the rpl5-rps14-cob gene cluster in pea mitochondria", MOL G GENET, 261(3), 1999, pp. 537-545

Abstract

In pea mitochondria the rpl5, rpsl4 and cob ORFs are clustered in a uniquegenomic environment and are cotranscribed into a 4.7-kb primary transcriptand several other polycistronic RNAs with sizes between 4.0 and 2.3 kb. All of the larger RNAs terminate at a common 3' end, 52 nucleotides downstream of the cob gene. Transcription is initiated at a promoter about 1.3 kb upstream of the rpl5 start codon. The promoter sequence 5'-AATAAGAGA-3' corresponds to the highly conserved 5'-CRTAAGAGA-3' motif often found in promoters in dicot plants. Functional analysis in a homologous in vitro transcription system showed the pea rpl5 promoter to be active, despite the presence of an altered base in first position of the promoter motif. In Oenothera, in contrast to pea, transcription of the rpl5 gene is driven by a promoter motif that conforms perfectly to the consensus sequence. Double inverted repeats located in the 3' flanking regions of the rpsl4 and cob ORFs in pea were investigated with respect to their possible role in defining transcript termini and their potential function in controlling exo- and endonucleolytic processing or transcript stabilization.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 14:37:42