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Titolo:
Reversible tumorigenesis induced by deficiency of vasodilator-stimulated phosphoprotein
Autore:
Liu, KY; Li, LM; Nisson, PE; Gruber, C; Jessee, J; Cohen, SN;
Indirizzi:
Stanford Univ, Med Ctr, Dept Genet, Stanford, CA 94305 USA Stanford Univ Stanford CA USA 94305 r, Dept Genet, Stanford, CA 94305 USA Stanford Univ, Med Ctr, Dept Med, Stanford, CA 94305 USA Stanford Univ Stanford CA USA 94305 Ctr, Dept Med, Stanford, CA 94305 USA Life Technol Inc, Rockville, MD 20850 USA Life Technol Inc Rockville MD USA 20850 hnol Inc, Rockville, MD 20850 USA
Titolo Testata:
MOLECULAR AND CELLULAR BIOLOGY
fascicolo: 5, volume: 19, anno: 1999,
pagine: 3696 - 3703
SICI:
0270-7306(199905)19:5<3696:RTIBDO>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-SUPPRESSOR GENE; RICH FOCAL ADHESION; HUMAN-PLATELETS; CHROMOSOME 19Q; OVARIAN-CANCER; CELL-ADHESION; ALPHA-ACTININ; PROTEIN VASP; RB GENE; GROWTH;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
70
Recensione:
Indirizzi per estratti:
Indirizzo: Cohen, SN Stanford Univ, Med Ctr, Dept Genet, Room M-320, Stanford, CA 94305 USA Stanford Univ Room M-320 Stanford CA USA 94305 ord, CA 94305 USA
Citazione:
K.Y. Liu et al., "Reversible tumorigenesis induced by deficiency of vasodilator-stimulated phosphoprotein", MOL CELL B, 19(5), 1999, pp. 3696-3703

Abstract

Random homozygous knockout (RHKO) is an antisense RNA strategy capable of identifying genes whose homozygous functional inactivation yields a selectable phenotype in cells growing in culture. Using this approach, we isolatedNIH 3T3 fibroblast clones that showed the ability to form colonies on 0.5%agar and tumors in nude mice, The gene inactivated in one of these clones was found to encode VASP (vasodilator-stimulated phosphoprotein), a previously identified protein that binds to components of the cadherin-catenin junctional complex and has been implicated in cell-cell interactions, the formation of actin filaments, and the transmission of signals at the cytoskeleton-membrane interface. Fibroblasts made deficient in VASP by RHKO showed loss of contact inhibition, and consequently, continued cell division past confluence. Restoration of VASP function by reversal of RHKO yielded cells that had lost the neoplastic capabilities acquired during RHKO. Overproduction of VASP mRNA in the sense or antisense orientation from expression constructs introduced by transfection into naive NM 3T3 fibroblasts also resultedin neoplastic transformation, implying that normal cell growth may requirethe maintenance of VASP expression within a narrow range, Our results implicate VASP in tumorigenesis and/or cancer progression.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 13:08:06