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Titolo:
High performance liquid chromatographic determination of free amino acids in algae
Autore:
Peter, A; Tourwe, D; Baumann, MEM; Elskens, M; Goeyens, L;
Indirizzi:
Attila Jozsef Univ, Dept Inorgan & Analyt Chem, H-6701 Szeged, Hungary Attila Jozsef Univ Szeged Hungary H-6701 yt Chem, H-6701 Szeged, Hungary Free Univ Brussels, Eenheid Organ Chem, B-1050 Brussels, Belgium Free UnivBrussels Brussels Belgium B-1050 hem, B-1050 Brussels, Belgium Alfred Wegener Inst Polar & Marine Res, D-27515 Bremerhaven, Germany Alfred Wegener Inst Polar & Marine Res Bremerhaven Germany D-27515 rmany Free Univ Brussels, Eenheid Analyt Chem, B-1050 Brussels, Belgium Free Univ Brussels Brussels Belgium B-1050 hem, B-1050 Brussels, Belgium
Titolo Testata:
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
fascicolo: 7, volume: 22, anno: 1999,
pagine: 1077 - 1093
SICI:
1082-6076(1999)22:7<1077:HPLCDO>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
9-FLUORENYLMETHYL CHLOROFORMATE DERIVATIVES; DISSOLVED ORGANIC NITROGEN; PRECOLUMN DERIVATIZATION; PHYTOPLANKTON;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Peter, A Attilagarysef Univ, Dept Inorgan & Analyt Chem, POB 440, H-6701 Szeged, Hun Attila Jozsef Univ POB 440 Szeged Hungary H-6701 701 Szeged, Hun
Citazione:
A. Peter et al., "High performance liquid chromatographic determination of free amino acids in algae", J LIQ CHR R, 22(7), 1999, pp. 1077-1093

Abstract

The amino acid compositions were determined in four different algal species: the temperate Thalassiosira rotula and Phaeocystis globosa and the polarThalassiosira antarctica and Phaeocystis antarctica. An extraction procedure was developed to obtain amino acids from phytoplankton species. The amino acids were analysed after precolumn derivatization with 9-fluorenylmethoxycarbonyl chloride (FMOC-CI); the procedure was optimized and the samples were analysed quantitatively on a Superspher RP8 C-8 column. The good stability of the FMOC derivatives was utilized to develop anew semipreparative chromatographic method for the separation and collection of the most important amino acids.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 04:11:11