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Titolo:
Amino acid analysis of peptides using HPLC with evaporative light scattering detection
Autore:
Peterson, JA; Lorenz, LJ; Risley, DS; Sandmann, BJ;
Indirizzi:
Eli Lilly & Co, Lilly Corp Ctr, Indianapolis, IN 46285 USA Eli Lilly & CoIndianapolis IN USA 46285 Ctr, Indianapolis, IN 46285 USA Butler Univ, Coll Pharm & Hlth Sci, Indianapolis, IN 46208 USA Butler Univ Indianapolis IN USA 46208 lth Sci, Indianapolis, IN 46208 USA
Titolo Testata:
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
fascicolo: 7, volume: 22, anno: 1999,
pagine: 1009 - 1025
SICI:
1082-6076(1999)22:7<1009:AAAOPU>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; FLAME IONIZATION DETECTION; PRECOLUMN DERIVATIZATION; PARATHYROID-HORMONE; LIVER-MICROSOMES; MASS DETECTOR; LIPID CLASSES; RAPID METHOD; SEPARATION; QUANTITATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Peterson, JA Eli Lilly & Co, Lilly Corp Ctr, Indianapolis, IN 46285 USA Eli Lilly & Co Indianapolis IN USA 46285 polis, IN 46285 USA
Citazione:
J.A. Peterson et al., "Amino acid analysis of peptides using HPLC with evaporative light scattering detection", J LIQ CHR R, 22(7), 1999, pp. 1009-1025

Abstract

A new procedure for the amino acid analysis of peptides has been devised utilizing high performance liquid chromatography (HPLC) with evaporative light scattering detection (ELSD). This procedure eliminates the need for complex derivatization schemes inherent of previous amino acid analysis procedures since the ELSD detects the amino acids directly. This quantitative method detects and separates 18 of the common amino acids in a one hour run time using cation exchange chromatography coupled with ELSD. The procedure wastested by analyzing the hydrolysate of human parathyroid hormone 1-34 (PTH), a synthetic polypeptide. A standard digestion consisting of 24 hour hydrolysis at 110 degrees C in 6 N hydrochloric acid with 3% phenol was used. Validation data reveal this is an accurate and precise procedure for the amino acid analysis of peptides.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 11:29:48