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Titolo:
Neuroinvasiveness of pseudorabies virus injected intracerebrally is dependent on viral concentration and terminal field density
Autore:
Card, JP; Enquist, LW; Moore, RY;
Indirizzi:
Univ Pittsburgh, Dept Neurosci, Pittsburgh, PA 15260 USA Univ Pittsburgh Pittsburgh PA USA 15260 eurosci, Pittsburgh, PA 15260 USA Univ Pittsburgh, Dept Psychiat, Pittsburgh, PA 15260 USA Univ Pittsburgh Pittsburgh PA USA 15260 sychiat, Pittsburgh, PA 15260 USA Univ Pittsburgh, Dept Neurol, Pittsburgh, PA 15260 USA Univ Pittsburgh Pittsburgh PA USA 15260 Neurol, Pittsburgh, PA 15260 USA Princeton Univ, Dept Mol Biol, Princeton, NJ 08544 USA Princeton Univ Princeton NJ USA 08544 t Mol Biol, Princeton, NJ 08544 USA
Titolo Testata:
JOURNAL OF COMPARATIVE NEUROLOGY
fascicolo: 3, volume: 407, anno: 1999,
pagine: 438 - 452
SICI:
0021-9967(19990510)407:3<438:NOPVII>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
HERPES-SIMPLEX VIRUS; CENTRAL-NERVOUS-SYSTEM; VENTRAL TEGMENTAL AREA; NIGRA PARS RETICULATA; RAT SUBSTANTIA-NIGRA; TRANSNEURONAL TRANSPORT; BASAL GANGLIA; PALLIDOSTRIATAL PROJECTION; SUPERIOR COLLICULUS; NEURONAL INFECTION;
Keywords:
transynaptic tracing; neurotropic virus; striatum;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
58
Recensione:
Indirizzi per estratti:
Indirizzo: Card, JP Univ Pittsburgh, Dept Neurosci, 446 Crawford Hall, Pittsburgh, PA15260 USA Univ Pittsburgh 446 Crawford Hall Pittsburgh PA USA 15260 260 USA
Citazione:
J.P. Card et al., "Neuroinvasiveness of pseudorabies virus injected intracerebrally is dependent on viral concentration and terminal field density", J COMP NEUR, 407(3), 1999, pp. 438-452

Abstract

Pseudorabies virus (PRV), a neurotropic swine a herpesvirus, has been usedextensively for transneuronal analysis of multisynaptic circuitry after peripheral injection. In the present analysis, we examined the influence of viral concentration and neuronal architecture on the invasiveness, replication, and transynaptic passage of an attenuated strain of PRV (PRV-Bartha) injected into rat striatum. Different concentrations of PRV-Bartha were injected into the striatum at a constant rate of infusion (10 nl/minute), and animals were killed 50 hours later. Viral concentration was manipulated by either altering the volume of the inoculum (100, 50, 20 nl) or by diluting the inoculum within a constant volume of 100 nl. Immunohistochemical localization of infected neurons revealed dramatic differences in the progression of infection that were dependent directly on the concentration of injected virus. In every case, the pattern of infection was consistent with preferential uptake of virions by axon terminals and retrograde transynaptic passageof virus from the injection site. The known topographically organized corticostriatal projections permitted a precise definition of the zone of viraluptake. This analysis demonstrated that the "effective zone of viral uptake" (i.e., the zone within which viral uptake led to productive replication of virus) varied in relation to the concentration of injected virus, with the highest concentration of PRV invading terminals within a 500 mu m radiusof the canula. Concentration-dependent changes in the progression of retrograde transynaptic infection also were observed. The highest concentration of virus produced the most extensive infection. The distribution of infected neurons in these cases included those with known afferent projections to striatum as well as those that became infected by retrograde transynaptic infection. Lesser concentrations of PRV-Bartha produced an increasingly restricted infection of the same circuitry within the same postinoculation interval. It is noteworthy that neurons known to elaborate dense striatal terminal fields were less sensitive to reduction in viral concentration than those giving rise to terminal fields of lesser density. Collectively, the dataindicate that the onset of viral replication after intracerebral injectionof PRV is directly dependent on virus concentration and terminal field density at the site of virus injection. J. Comp. Neurol. 1999;407:438-452. (C)1999 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 06:43:19