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Titolo:
Cloning of murine translation initiation factor 6 and functional analysis of the homologous sequence YPR016c in Saccharomyces cerevisiae
Autore:
Wood, LC; Ashby, MN; Grunfeld, C; Feingold, KR;
Indirizzi:
Dept Vet Affairs Med Ctr, Dermatol Serv, San Francisco, CA 94121 USA Dept Vet Affairs Med Ctr San Francisco CA USA 94121 ancisco, CA 94121 USA Dept Vet Affairs Med Ctr, Med Serv, San Francisco, CA 94121 USA Dept Vet Affairs Med Ctr San Francisco CA USA 94121 ancisco, CA 94121 USA Univ Calif San Francisco, Dept Dermatol, San Francisco, CA 94121 USA Univ Calif San Francisco San Francisco CA USA 94121 ancisco, CA 94121 USA Univ Calif San Francisco, Dept Med, San Francisco, CA 94121 USA Univ CalifSan Francisco San Francisco CA USA 94121 ancisco, CA 94121 USA Acacia Biosci Inc, Richmond, CA 94806 USA Acacia Biosci Inc Richmond CA USA 94806 iosci Inc, Richmond, CA 94806 USA
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 17, volume: 274, anno: 1999,
pagine: 11653 - 11659
SICI:
0021-9258(19990423)274:17<11653:COMTIF>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
REV ACTIVATION DOMAIN; CAP-BINDING PROTEIN; CELL-PROLIFERATION; SUBUNIT BIOGENESIS; BARRIER DISRUPTION; GROWTH-FACTOR; DNA-SYNTHESIS; YEAST; GENE; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Wood, LC Depto,et Affairs Med Ctr, Dermatol Serv, 4150 Clement St,111F, San Francisc Dept Vet Affairs Med Ctr 4150 Clement St,111F San Francisco CA USA 94121
Citazione:
L.C. Wood et al., "Cloning of murine translation initiation factor 6 and functional analysis of the homologous sequence YPR016c in Saccharomyces cerevisiae", J BIOL CHEM, 274(17), 1999, pp. 11653-11659

Abstract

The cDNA sequence of a murine gene whose expression was up-regulated afterepidermal injury was cloned utilizing differential display. The full-length cDNA was isolated by 3' and 5' rapid amplification of cDNA ends from mouse liver. The predicted protein is >97% identical to the human sequence for eukaryotic translation initiation factor (eIF) 6, thus identifying the geneas murine eIF6, Functional studies of the yeast eIF6 homolog, YPR016c, were initiated in Saccharomyces cerevisiae to determine the cellular role(s) of eIF6. Complete deletion of the YPR016c coding sequence was lethal. Viability was restored in the presence of either YPR016c of murine eIF6, when either was expressed as aminoterminal green fluorescent protein fusion protein. Moreover, both fusion proteins localized to nuclear/perinuclear compartments in their respective yeast strains. When the expression of YPR016c-greenfluorescent protein was repressed, there was a dramatic reduction in the 60 S ribosomal subunit and polysome content and decreased 80S monosome content. Additionally, the YPR016c-depleted cells arrested in G(1). These studies show that YPR016c, which encodes yeast eIF6, is necessary for maximal polysome formation and plays an important role in determining free 60 S ribosomal subunit content.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 12:14:49