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Titolo:
Interaction of SeqA and Dam methylase on the hemimethylated origin of Escherichia coli chromosomal DNA replication
Autore:
Kang, S; Lee, H; Han, JS; Hwang, DS;
Indirizzi:
Seoul Natl Univ, Inst Mol Biol & Genet, Seoul 151742, South Korea Seoul Natl Univ Seoul South Korea 151742 enet, Seoul 151742, South Korea Seoul Natl Univ, Dept Microbiol, Seoul 151742, South Korea Seoul Natl Univ Seoul South Korea 151742 biol, Seoul 151742, South Korea SeoulKorea Univ, Interdisciplinary Program Genet Engn, Seoul 151742, SouthSeoul Natl Univ Seoul South Korea 151742 Genet Engn, Seoul 151742, South
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 17, volume: 274, anno: 1999,
pagine: 11463 - 11468
SICI:
0021-9258(19990423)274:17<11463:IOSADM>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
MISMATCH CORRECTION; INITIATION; PROTEIN; METHYLTRANSFERASE; GENE; PROMOTER; MUTANTS; EXPRESSION; SEQUENCE; PREVENTS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Hwang, DS Seoul Natl Univ, Inst Mol Biol & Genet, Seoul 151742, South Korea Seoul Natl Univ Seoul South Korea 151742 l 151742, South Korea
Citazione:
S. Kang et al., "Interaction of SeqA and Dam methylase on the hemimethylated origin of Escherichia coli chromosomal DNA replication", J BIOL CHEM, 274(17), 1999, pp. 11463-11468

Abstract

Preferential binding of SeqA protein to hemimethylated oriC, the origin ofEscherichia coli chromosomal replication, delays methylation by Dam methylase. Because the SeqA-oriC interaction appears to be essential in timing ofchromosomal replication initiation, the biochemical functions of SeqA protein and Dam methylase at the 13-mer L, M, and R region containing 4 GATC sequences at the left end of oriC were examined. We found that SeqA protein preferentially bound hemimethylated 13-mers butnot fully nor unmethylated 13-mers. Regardless of strand methylation, the binding of SeqA protein to the hemimethylated GATC sequence of 13-mer L wasfollowed by additional binding to other hemimethylated GATC sequences of 13-mer M and R. On the other hand, Dam methylase did not discriminate binding of 13-mers in different methylation patterns and was not specific to GATCsequences. The binding specificity and higher affinity of SeqA protein over Dam methylase to the hemimethylated 13-mers along with the reported cellular abundance of this protein explains the dominant action of SeqA protein over Dam methylase to the newly replicated oriC for the sequestration of chromosomal replication. Furthermore, SeqA protein bound to hemimethylated 13-mers was not dissociated by Dam methylase, and most SeqA protein spontaneously dissociated 10 min after binding. Also, SeqA protein delayed the in vitro methylation of hemimethylated 13-mers by Dam methylase. These in vitro results suggest that the intrinsic binding instability of SeqA protein results in release of sequestrated hemimethylated oriC.

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Documento generato il 05/04/20 alle ore 18:39:36