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Titolo:
Characterization of the 70-kDa peroxisomal membrane protein, an ATP binding cassette transporter
Autore:
Imanaka, T; Aihara, K; Takano, T; Yamashita, A; Sato, R; Suzuki, Y; Yokota, S; Osumi, T;
Indirizzi:
Toyama00194, Pharmaceut Univ, Fac Pharmaceut Sci, Dept Biol Chem, Toyama 93 Toyama Med & Pharmaceut Univ Toyama Japan 9300194 t Biol Chem, Toyama 93 Teikyo145,v, Fac Pharmaceut Sci, Dept Microbiol & Mol Pathol, Kanagawa 1990 Teikyo Univ Kanagawa Japan 1990145 Microbiol & Mol Pathol, Kanagawa 1990 Teikyopaniv, Fac Pharmaceut Sci, Dept Hyg Chem & Nutr, Kanagawa 1990145, Ja Teikyo Univ Kanagawa Japan 1990145 Hyg Chem & Nutr, Kanagawa 1990145, Ja Osaka Univ, Grad Sch Pharmaceut Sci, Biochem & Mol Biol Lab, Osaka 5650871, Osaka Univ Osaka Japan 5650871 i, Biochem & Mol Biol Lab, Osaka 5650871, Gifu Univ, Sch Med, Dept Pediat, Gifu 5008076, Japan Gifu Univ Gifu Japan 5008076 , Sch Med, Dept Pediat, Gifu 5008076, Japan Yamanashi Med Univ, Biol Lab, Yamanashi 4903898, Japan Yamanashi Med UnivYamanashi Japan 4903898 Lab, Yamanashi 4903898, Japan Himeji Inst Technol, Dept Life Sci, Kamigori, Hyogo 6781297, Japan Himeji Inst Technol Kamigori Hyogo Japan 6781297 ri, Hyogo 6781297, Japan
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 17, volume: 274, anno: 1999,
pagine: 11968 - 11976
SICI:
0021-9258(19990423)274:17<11968:COT7PM>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
X-LINKED ADRENOLEUKODYSTROPHY; CLOFIBRATE-TREATED RATS; FATTY-ACID OXIDATION; SACCHAROMYCES-CEREVISIAE; ABC-TRANSPORTER; BETA-OXIDATION; 3-KETOACYL-COA THIOLASE; ZELLWEGER-SYNDROME; LIVER PEROXISOMES; SIGNAL PEPTIDE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Imanaka, T Toyamatani,& Pharmaceut Univ, Fac Pharmaceut Sci, Dept Biol Chem, 2630 Sugi Toyama Med & Pharmaceut Univ 2630 Sugitani Toyama Japan 9300194
Citazione:
T. Imanaka et al., "Characterization of the 70-kDa peroxisomal membrane protein, an ATP binding cassette transporter", J BIOL CHEM, 274(17), 1999, pp. 11968-11976

Abstract

The 70-kDa peroxisomal membrane protein (PMP70) is one of the major components of rat liver peroxisomal membranes and belongs to a superfamily of proteins known as ATP binding cassette transporters. PMP70 is markedly inducedby administration of hypolipidemic agents in parallel with peroxisome proliferation and induction of peroxisomal fatty acid beta-oxidation enzymes, To characterize the role of PMP70 in biogenesis and function of peroxisomes,we transfected the cDNA of rat PMP70 into Chinese hamster ovary cells and established cell lines stably expressing PMP70. The content of PMP70 in thetransfectants increased about 5-fold when compared with the control cells. A subcellular fractionation study showed that overexpressed PMP70 was enriched in peroxisomes, This peroxisomal localization was confirmed by immunofluorescence and immunoelectron microscopy. The number of immune-gold particles corresponding to PMP70 on peroxisomes increased markedly in the transfectants, but the size and the number of peroxisomes were essentially the same in both the transfectants and the control cells. beta-Oxidation of palmitic acid increased about 2-3-fold in the transfectants, whereas the oxidation of lignoceric acid decreased about 30-40%. When intact peroxisomes prepared from both the cell lines were incubated with palmitoyl-CoA, oxidation was stimulated with ATP, but the degree of the stimulation was higher in the transfectants than in the control cells. Furthermore, we established three Chinese hamster ovary cell lines stably expressing mutant PMP70. In these cells, beta-oxidation of palmitic acid decreased markedly, These results suggest that PMP70 is involved in metabolic transport of long chain acyl-CoA across peroxisomal membranes and that increase of PMP70 is not associated with proliferation of peroxisomes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 18:24:26