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Titolo:
Interaction of neuronal nitric-oxide synthase with alpha 1-syntrophin in rat brain
Autore:
Hashida-Okumura, A; Okumura, N; Iwamatsu, A; Buijs, RM; Romijn, HJ; Nagai, K;
Indirizzi:
Osaka Univ, Inst Prot Res, Div Prot Metab, Osaka 5650871, Japan Osaka Univ Osaka Japan 5650871 Res, Div Prot Metab, Osaka 5650871, Japan Kirin Brewery Co Ltd, Cent Labs Key Technol, Kanazawa Ku, Kanagawa 2360004, Kirin Brewery Co Ltd Kanagawa Japan 2360004 nazawa Ku, Kanagawa 2360004, Netherlands Inst Res, NL-1105 AZ Amsterdam Zuidoost, Netherlands Netherlands Inst Res Amsterdam Zuidoost Netherlands NL-1105 AZ therlands
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 17, volume: 274, anno: 1999,
pagine: 11736 - 11741
SICI:
0021-9258(19990423)274:17<11736:IONNSW>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
SKELETAL-MUSCLE; NERVOUS-SYSTEM; DYSTROPHIN; LOCALIZATION; SYNTROPHIN; PROTEIN; COLOCALIZATION; RELEASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Hashida-Okumura, A Osakaapanv, Inst Prot Res, Div Prot Metab, 3-2 Yamada Oka, Osaka 5650871, J Osaka Univ 3-2 Yamada Oka Osaka Japan 5650871 50871, J
Citazione:
A. Hashida-Okumura et al., "Interaction of neuronal nitric-oxide synthase with alpha 1-syntrophin in rat brain", J BIOL CHEM, 274(17), 1999, pp. 11736-11741

Abstract

Neuronal nitric-oxide synthase (nNOS) has a PSD-95/ Dlg/ZO-1 (PDZ) domain that can interact with multiple proteins. nNOS has been known to interact with PSD-95 and a related protein, PSD-93, in brain and with alpha 1-syntrophin in skeletal muscle in mammals. In this study, we have purified an nNOS-interacting protein from bovine brain using an affinity column made of Sepharose conjugated with glutathione S-transferase-rat nNOS fusion protein andidentified it as alpha 1-syntrophin by microsequencing. Immunostaining of primary cultures of rat embryonic brain neuronal cells with antibodies against these proteins showed that nNOS and alpha 1-syntrophin were colocalizedin neuronal cell bodies and neurites. Immunohistochemical analysis indicated that the nNOS- and alpha 1-syntrophin-like immunoreactive substances were highly expressed in the rat hypothalamic suprachiasmatic nucleus (SCN) and paraventricular nucleus. In the SCN, nNOS- and alpha 1-syntrophin-like immunoreactive substances were colocalized in the same neurons as detected byconfocal microscopy. These results indicate that nNOS in brain interacts with alpha 1-syntrophin in specific neurons of the SCN and paraventricular nucleus and that this interaction might play a physiological role in functions of these neurons.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 02:52:28