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Titolo:
Differential expression of alpha v integrins in K1735 melanoma cells
Autore:
Li, XW; Chen, B; Blystone, SD; McHugh, KP; Ross, FP; Ramos, DM;
Indirizzi:
Univ Calif San Francisco, Dept Stomatol, San Francisco, CA 94143 USA Univ Calif San Francisco San Francisco CA USA 94143 ancisco, CA 94143 USA SUNY Hlth Sci Ctr, Dept Anat, Syracuse, NY 13210 USA SUNY Hlth Sci Ctr Syracuse NY USA 13210 Dept Anat, Syracuse, NY 13210 USA SUNY Hlth Sci Ctr, Dept Cell Biol, Syracuse, NY 13210 USA SUNY Hlth Sci Ctr Syracuse NY USA 13210 Cell Biol, Syracuse, NY 13210 USA Washington Univ, Sch Med, Dept Pathol, St Louis, MO 63110 USA Washington Univ St Louis MO USA 63110 Dept Pathol, St Louis, MO 63110 USA
Titolo Testata:
INVASION & METASTASIS
fascicolo: 1, volume: 18, anno: 1998,
pagine: 1 - 14
SICI:
0251-1789(199801/02)18:1<1:DEOAVI>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR PROGRESSION; ADHESION; VITRONECTIN; ALPHA(V)BETA(3); RECEPTOR; PHOSPHORYLATION; ASSOCIATION; REQUIREMENT; INVOLVEMENT; LINES;
Keywords:
differential expression, alpha v integrins; extracellular matrix; melanoma cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Ramos, DM UnivACalif San Francisco, Dept Stomatol, HSW 604,Box 0512, San Francisco, C Univ Calif San Francisco HSW 604,Box 0512 San Francisco CA USA 94143
Citazione:
X.W. Li et al., "Differential expression of alpha v integrins in K1735 melanoma cells", INVAS METAS, 18(1), 1998, pp. 1-14

Abstract

Tumor cell adherence to and migration on the extracellular matrix is an important aspect of cancer progression. This interaction with the extracellular matrix is mediated primarily through the integrin class of cell adhesionmolecules. We identified a restricted expression of alpha v beta 3 in highly metastatic K1735M2 and of alpha v beta 5 in poorly metastatic K1735C23 murine melanoma cells. The highly metastatic cells were ten times more motile on vitronectin and fibronectin and approximately three times more invasive through a reconstituted basement membrane than the poorly metastatic cells. This motility was inhibited by addition of anti-beta 3 antibodies. Injection of the alpha v beta 3-negative K1735C23 cells into syngeneic mice resulted in the generation of a metastatic variant (K1735C23PM) that neo expressed the alpha v beta 3 complex, indicating that expression of alpha v beta 3 is required for K1735 melanoma metastasis. Injection of highly metastaticK1735M2 cells in the presence of blocking antibody to beta 3 reduced tumorsize by approximately 80%. Treatment of the K1735M2 cells with a retroviral antisense beta 3 construct significantly reduced their expression of alpha v beta 3 and also reduced their motility on extracellular matrix ligands and their invasion through a reconstituted basement membrane. In contrast, when the K1735C23 cells were treated with a construct containing the full-length beta 3 cDNA, their motility on extracellular matrix proteins and invasion of at reconstituted basement membrane were significantly increased. These results indicate that alpha v beta 3 is required for migration and invasion of K1735 melanoma cells in vitro and primary tumor growth and metastasis in vivo.

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Documento generato il 04/07/20 alle ore 21:23:43