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Titolo:
Localization of HArep-containing genes on the chromosome of Porphyromonas gingivalis W83
Autore:
Lewis, JP; Macrina, FL;
Indirizzi:
Virginia Commonwealth Univ, Inst Oral & Craniofacial Mol Biol, Richmond, VA Virginia Commonwealth Univ Richmond VA USA 23298 l Mol Biol, Richmond, VA
Titolo Testata:
INFECTION AND IMMUNITY
fascicolo: 5, volume: 67, anno: 1999,
pagine: 2619 - 2623
SICI:
0019-9567(199905)67:5<2619:LOHGOT>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
ARGININE-SPECIFIC PROTEASE; PROTEINASE LYS-GINGIPAIN; CYSTEINE PROTEINASE; ARG-GINGIPAIN; BACTEROIDES-GINGIVALIS; PERIODONTAL-DISEASE; MOLECULAR-CLONING; MATRIX PROTEINS; ENCODING GENES; DIRECT REPEATS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Macrina, FL Virginia Commonwealth Univ, Inst Oral & Craniofacial Mol Biol,Richmond, VA Virginia Commonwealth Univ Richmond VA USA 23298 Richmond, VA
Citazione:
J.P. Lewis e F.L. Macrina, "Localization of HArep-containing genes on the chromosome of Porphyromonas gingivalis W83", INFEC IMMUN, 67(5), 1999, pp. 2619-2623

Abstract

We have mapped a group of virulence genes of Porphyromonas gingivalis to asingle large fragment of the genome. These genes (rgpA, kgp, and hagA) allcontain a consensus repeat sequence (HArep). rgpA and kgp encode cysteine proteases with Arg-X and Lys-X specificity, respectively, and hagA encodes a hemagglutinin, Genomic DNA fragments separated by pulse-field gel electrophoresis were blotted and probed in order to localize the genes to a 0.25-Mb NheI fragment of the P. gingivalis W83 genome, Further hybridization analyses with single-and double-restriction digestion allowed us to generate a physical map of the fragment and determine the precise locations of the protease and hemagglutinin genes, In addition, we found an insertion-like sequence, IS195, near the ends of the 0.25-Mb NheI fragment. A similarly sized fragment carrying HArep sequences was also demonstrated in the P. gingivalis W12 and W50 genomes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 07:41:30