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Titolo:
Proliferative behavior of the murine cerebral wall in tissue culture: Cellcycle kinetics and checkpoints
Autore:
Takahashi, T; Bhide, PG; Goto, T; Miyama, S; Caviness, VS;
Indirizzi:
Harvard4Univ, Sch Med, Massachusetts Gen Hosp, Dept Neurol, Boston, MA 0211 Harvard Univ Boston MA USA 02114 s Gen Hosp, Dept Neurol, Boston, MA 0211 Keio Univ, Sch Med, Dept Pediat, Tokyo 160, Japan Keio Univ Tokyo Japan 160 o Univ, Sch Med, Dept Pediat, Tokyo 160, Japan
Titolo Testata:
EXPERIMENTAL NEUROLOGY
fascicolo: 2, volume: 156, anno: 1999,
pagine: 407 - 417
SICI:
0014-4886(199904)156:2<407:PBOTMC>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
NEOCORTICAL NEURONOGENESIS; DROSOPHILA EMBRYOGENESIS; VENTRICULAR ZONE; G1 PHASE; EPITHELIUM; NEURONS; MARKER; GLUTAMATE; PROTEIN; MODEL;
Keywords:
neocortical development; cell cycle; mouse; organotypic explants; tissue culture;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Takahashi, T Harvard4Univ, Sch Med, Massachusetts Gen Hosp, Dept Neurol, Boston, MA 0211 Harvard Univ Boston MA USA 02114 ept Neurol, Boston, MA 0211
Citazione:
T. Takahashi et al., "Proliferative behavior of the murine cerebral wall in tissue culture: Cellcycle kinetics and checkpoints", EXP NEUROL, 156(2), 1999, pp. 407-417

Abstract

Cerebral wall from embryonic day 13 mice was cultured in a three-dimensional collagen matrix in defined, serum-free medium. The cerebral wall retained its normal architecture, including the radial glial fiber system, for up to 19 h in culture. The cell cycle was initially blocked at the S/G2/M and the G1/S phase transitions, resulting in a transient synchronization of theproliferative cells. The transient blockades correspond, we suggest, to the G2 checkpoint and G1 restriction point, adaptive mechanisms of normal proliferative cells. The blocks were relieved within a few hours of explantation with restoration of the interkinetic nuclear migration and flow of cellsthrough the cycle phases. The duration of the reestablished cell cycle andthose of G1, S, and combined G2-M phases were estimated to be 19.2, 6.3-8.3, 8.8, and 2.0-4.0 h, respectively. The leaving (Q) fraction of the cycle (0.64) was twice the in vivo value. Two-thirds of the Q fraction cells remained in the ventricular epithelium, resulting in a substantially low growthfraction of 0.73 compared with 1.0 in vivo. The embryonic murine cerebral explant, cultured in minimum essential medium, should be favorable for studies of cycle modulatory actions of cell external influences such as growth factors or neurotransmitters. (C) 1999 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 07:52:30