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Titolo:
ADENOASSOCIATED VIRUS (AAV) VECTOR ANTISENSE GENE-TRANSFER IN-VIVO DECREASES GABA(A) ALPHA(1) CONTAINING RECEPTORS AND INCREASES INFERIOR COLLICULAR SEIZURE SENSITIVITY
Autore:
XIAO X; MCCOWN TJ; LI J; BREESE GR; MORROW AL; SAMULSKI RJ;
Indirizzi:
UNIV N CAROLINA,GENE THERAPY CTR,CB 7352,7119 THURSTON BLDG CHAPEL HILL NC 27599 UNIV N CAROLINA,GENE THERAPY CTR CHAPEL HILL NC 27599 UNIV N CAROLINA,CTR NEUROSCI CHAPEL HILL NC 27599 UNIV N CAROLINA,CTR ALCOHOL STUDIES CHAPEL HILL NC 27599 UNIV N CAROLINA,DEPT PHARMACOL CHAPEL HILL NC 27599 UNIV N CAROLINA,DEPT PSYCHIAT CHAPEL HILL NC 27599 SOMATIX CORP CHAPEL HILL NC 00000
Titolo Testata:
Brain research
fascicolo: 1-2, volume: 756, anno: 1997,
pagine: 76 - 83
SICI:
0006-8993(1997)756:1-2<76:AV(VAG>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAT-BRAIN; BINDING-SITES; MESSENGER-RNA; EXPRESSION; SUBUNITS;
Keywords:
ADENOASSOCIATED VIRUS; GENE THERAPY; INFERIOR COLLICULUS; SEIZURE; GAMMA-AMINOBUTYRIC ACID (GABA); ANTISENSE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
X. Xiao et al., "ADENOASSOCIATED VIRUS (AAV) VECTOR ANTISENSE GENE-TRANSFER IN-VIVO DECREASES GABA(A) ALPHA(1) CONTAINING RECEPTORS AND INCREASES INFERIOR COLLICULAR SEIZURE SENSITIVITY", Brain research, 756(1-2), 1997, pp. 76-83

Abstract

In the inferior colliculus, adeno-associated virus (AAV) vectors are capable of gene transfer and stable, long-term expression, but it remained to be shown if this in vivo gene transfer could alter focal seizure sensitivity in the inferior colliculus. Because GABA receptors directly modulate inferior collicular seizures, AAV vectors were constructed with a cytomegalovirus (CMV) promoter and a truncated, human GABA(A) alpha(1), cDNA in both the sense and antisense orientations. Seven days after collicular microinjection of the sense vectors (1 mu l; 3 x 10(9) particles/mu l), neurons exhibited GABA(A) alpha-like immunoreactivity in amounts far exceeding endogenous concentrations. Unilateral or bilateral sense vector infusion had no effect on inferior collicular seizure parameters or on [H-3]zolpidem binding. In contrast, bilateral infusion of the antisense AAV-GABA(A) alpha(1) vector (1 mu l; 3 x 10(8) particles/mu l) caused a 137% increase in the seizure duration. Moreover, unilateral antisense vector infusion produced a localized, 48% decrease in [H-3]zolpidem binding. Thus, in the inferior colliculus, antisense AAV-CMV vectors can reduce a specific receptor subunit protein and change receptor function that directly influences in vivo seizure sensitivity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/08/20 alle ore 16:16:34